中国比较医学杂志2016，Vol.26Issue(7)：42-47,6.DOI:10.3969.j.issn.1671-7856.2016.07.007
丙泊酚干预对大鼠视神经损伤的影响及机制研究
NeuroProtective mechanisms of ProPofol to retinal ganglion cells in a Partial oPtic nerve crush rat model
李学东 1陈家康 1覃军 1麦用军 1肖振勇1
作者信息
- 1. 柳州市工人医院，广西 柳州 545000
- 折叠
摘要
Abstract
Objective To investigate the Protective effect of propofol on the retinal ganglion cells of the rat optic nerve crush model. Methods 67 SD rats. Randomly selected 20 rats, don’ t do any processing for the normal group. With more than 47 rats optic clamps for optic nerve contusion model, legal system building 5 failure, success of 42 rats were randomly divided into the optic nerve damage and propofol group, 21 / group. Will not be any treatment after optic nerve injury group building, 4 hours after propofol group for propofol therapy. After 4 days of successful modeling, the apoptosis of retinal ganglion cells were detected by TUNEL staining. After 7 days of successful modeling, the expression of Caspase⁃3, BCL⁃2 in retina and optic nerve cells of rats were detected by RT⁃PCR and Western⁃blot. After 14 days of successful modeling, The amplitude and latent period of P1 wave of flash visual evoked potential were detected. The animals were sacrificed and the optic nerve was taken to observe the pathological morphology of retina and optic nerve in rats, and the retinal ganglion cells ( RGC) were counted. Results After 4 days of successful modeling,the apoptosis of the propofol group was significantly lower than that of the optic nerve injury group (P < 0. 05). After 7 days of successful modeling,the expression of Caspase⁃3 in the propofol group was significantly lower than that in the propofol group ( P < 0. 05 ) , the expression of BCL⁃2 in the propofol group was significantly higher than that in the propofol group ( P < 0. 05 ) . After 14 days of successful modeling, FG Positive RGC numbers: normal group >propofol group > optic nerve injury group and between groups difference was statistically significant (P < 0. 05). The flash visual evoked potential of the rats in propofol group was significantly shorter than that in the optic nerve injury group (P < 0. 05) and the amplitude of the visual evoked Potential was significantly higher than that of the optic nerve injury group ( P < 0. 05 ) . Conclusions Propofol treatment can through an early reduction in rats after optic nerve crush RGCs apoptosis, decreased Caspase⁃3 expression, increased BCL⁃2 expression and improve number of optic nerve crush of RGCs survival.关键词
大鼠/视神经损伤/丙泊酚/视网膜神经节细胞/Caspase-3/BCL-2Key words
Rats/Optic nerve damage/Propofol/Retinal ganglion cells/Caspase-3/BCL-2分类
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李学东,陈家康,覃军,麦用军,肖振勇..丙泊酚干预对大鼠视神经损伤的影响及机制研究[J].中国比较医学杂志,2016,26(7):42-47,6.
