摘要
Abstract
Objective To investigate the role of small RNA 195 ( MicroRNA⁃137 ) , TGF⁃β1/Smads signal transduction pathway and angiotensin II ( Ang II ) in cardiac remodeling in spontaneously hypertensive rats ( SHR ) . Methods 16 SHR male rats were randomly divided into intervention group SHR ( captopril 10 mg/kg·d) and SHR control group ( distilled water) , the other 8 Wistar rats were normal control group, rats were given captopril 10 mg/kg·d or distilled water for 8 weeks. Caudal arterial pressure was measured before and after the intervention, intervention after 8 weeks rats were killed by exsanguination, HE staining was used to observe the morphological changes of rat heart, qRT⁃PCR method was used to detect the expression of miRNA⁃137 in rat heart, Western⁃blot detection of TGF⁃β1 and Ang II, Smad 3, Col⁃Ⅰand Col⁃Ⅲ protein. Results Compared to the normal control groups,the miRNA⁃137,AngⅡ,TGF⁃β1, Smad3,Col⁃Ⅰand Col⁃Ⅲ were higher expressed in SHR treatment group and SHR control groups(P<0. 01 or P<0. 05);Compared to the SHR control group,the cardiomyocyte of SB group becomes smaller and arranged more closely and orderly, the miRNA⁃137,AngⅡ,TGF⁃β1,Smad3,Col⁃Ⅰand Col⁃Ⅲ were significantly lower expressed(P<0. 01 or P<0. 05). Conclusions MiRNA⁃137 may promote SHR cardiac remodeling by up regulation of Ang II and TGF⁃β1/Smads signaling pathway;the captopril intervention can inhibit miRNA⁃137 expression.关键词
心脏重构/miRNA-137/自发性高血压大鼠/TGF-β1/Smads信号通路Key words
Heart Remodeling/miRNA-137/SHR/TGF-β1/Smads signaling pathway分类
医药卫生
