中国兽医杂志2016，Vol.50Issue(6)：9-15,7.
ADV 分离强毒株全基因突变重组质粒的构建、表达及其免疫原性的分析
ADV Isolated Virulent Strain Construction Expression and Immunogenicity Analysis of Whole Gene Mutation Recombinant Plasmids
摘要
Abstract
For the development of mink Aleutian disease nucleic acid vaccine,the nucleotide sequences encoding 428 ~446 bit amino acids in the ADV VP2 gene are removed by using the overlap extension PCR technique,with pcDNA3.1 (+)carrier connection,construct the whole gene mutation recombinant plasmid pcDNA3.1 -ADV -428,on this basis,nucleotide sequences of 487 ~501 amino acid at position is truncated coding,construct the whole gene mutation recombinant plasmid pcDNA3.1 -ADV -428 -487.The recombinant plasmid was constructed by intramuscular injection of immune mice,anti ADV antibody levels were detected in 14 days,28 days,42 days,and 56 days after inoculation by indirect ELISA method;Detection of CD3 +,CD4 + and CD8 +T lymphocyte subsets in spleen cells of mice forty -second days after inoculation by flow cytometry.Results showed that the number of CD3 +,CD4 + and CD8 + T lymphocyte subsets in mice inoculated with plasmid were significantly increased,anti ADV antibody level reached peak at forty -second days.In this experiment,the immunogenicity of the recombinant plasmid of ADV gene mutation was tested to provide reference for the development of mink Aleutian disease nucleic acid vaccine.关键词
水貂阿留申病毒/真核表达载体/反应原性/免疫原性Key words
aleutian disease of mink/eukaryotic expression vector/immunogenicity/reactionogenicity分类
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孙利杰,刘东旭,李健明,时坤,冷雪,李东,杜锐..ADV 分离强毒株全基因突变重组质粒的构建、表达及其免疫原性的分析[J].中国兽医杂志,2016,50(6):9-15,7.基金项目
国家自然基金 （）
