中国兽医杂志2016，Vol.50Issue(6)：16-21,6.
副结核分枝杆菌 MAP0862、MAP1345基因的串联表达及其在间接 ELISA 中的初步应用
Expression of Mycobacterium avium subsp.paratuberculosis MAP0862,MAP1345 and Primary Application in Indirect ELISA
摘要
Abstract
To explore the effect of MAP0862 and MAP1345 in the diagnosis of Bovine paratuberculosis,the specific protein acquired by tandem expression was used as coating antigen after purified and quantification,an indirect ELISA was preliminary established after a series of optimization.The specificity of this method was verified by MAP antisera,Brucella antisera,Bovine tuberculosis (TB) antisera,BCG antisera and negative sera.The detection result of 300 sera showed that the coincidence rate of MAP0862 -1345 indirect ELISA with commercial kit was 92.7%.关键词
副结核分枝杆菌/串联表达/间接 ELISAKey words
Mycobacterium avium subsp.paratuberculosis/tandem expression/indirect ELISA分类
农业科技引用本文复制引用
张振,张阁,彭永,彭小薇,孙翠丽,常维山,朱良全,丁家波..副结核分枝杆菌 MAP0862、MAP1345基因的串联表达及其在间接 ELISA 中的初步应用[J].中国兽医杂志,2016,50(6):16-21,6.基金项目
北京市科技新星计划（xx2013099）；现代农业人才支撑计划项目 （）
