中国兽医杂志2016，Vol.50Issue(6)：33-38,6.
氨苄西林在鸡排泄物中的残留消除规律
Ampxicillin Residue Depletion in Chicken Excrement
摘要
Abstract
A study on Ampxicillin (AMP)residue depletion was conducted in chicken excrement.AMP was deproteinized from chicken excrement with acetonitrile,the analyte was extracted by saturated dichloromethane. The supernatant was reacted with salicylaldehyde under acidic and boiling conditions.Finally,AMP was determinated by high performance liquid chromatography (HPLC)with fluorescence detector.The excitation wavelength was set at 354 nm and emission wavelength was set at 445 nm.The average recovery in chicken excrement was 74.96 % ~80.41 % with coefficients of variation (CV)lower than 9.77 %.The limit of detection (LOD)was 1.2 μg/kg(S /N ≥ 3)and the limit of quantitation (LOQ)was 3.5 μg/kg(S /N ≥ 10). After the chickens were orally administered successively AMP of 60.0 mg/kg and 120.0 mg/kg of body weight one time every day for 5 days,AMP residues in chicken excrements was detected at the first day,the maximum residues of AMP in chicken excrement were at the 5th day.Normal dose group,AMP residues in chicken excrement was lower than LOD at the 7th withdrawal day.Double dose group,AMP residues in chicken excrement was lower than LOD at the 9th withdrawal day.The residues of AMP in chicken excrement were all positively correlated with AMP orally administered doses.There are environmental risks with high residues of AMP in chicken excrement.关键词
氨苄西林/高效液相色谱荧光检测法/残留消除/鸡排泄物Key words
Ampxicillin/HPLC with fluorescence detection/residue depletion/chicken excrement分类
农业科技引用本文复制引用
王波,王金玉,张杨杨,谢恺舟,刘建宇,刘亚楠,高强,郭辉生,张跟喜,戴国俊..氨苄西林在鸡排泄物中的残留消除规律[J].中国兽医杂志,2016,50(6):33-38,6.基金项目
国家肉鸡产业技术体系专项（CARS －42－G23）；江苏高校优势学科建设工程资助项目（201410）；扬州大学＂新世纪人才工程＂资助项目 （）
