中国畜牧兽医2016，Vol.43Issue(7)：1667-1673,7.DOI:10.16431/j.cnki.1671-7236.2016.07.002
鲁西黄牛MSTN基因上游序列多态性对转录表达的影响
Effect of MSTN Gene Upstream Sequence Polymorphism on Transcriptional Expression in Luxi Huang Cattle
摘要
Abstract
Myostain (MSTN)gene is a member of transforming growth factor-β(TGF-β)super-family.In order to study the relationship between Luxi Huang cattle MSTN gene upstream se-quence polymorphism and the transcriptional expression,DNA was extracted from leg muscle cells of Luxi Huang cattle and then sequenced.After analyzing MSTN upstream sequence,an ev-olutionary tree was constructed and a SNP site was found at 805 bp of MSTN upstream sequence of initiation codon.Two expression vectors contained the SNP site were constructed to transfect the C2C12 cells invitro to study the influence of MSTN gene upstream sequence polymorphism on transcription activity.The results showed that single nucleotide change on MSTN gene upstream sequence affected the expression.It suggested that MSTN gene upstream sequence pol-ymorphism of Luxi Huang cattle would affect transcription activity and play an important role in gene expression regulation.关键词
MSTN基因/上游序列/单核苷酸多态性/转录表达Key words
MSTN gene/upstream sequence/single nucleotide polymorphism/transcriptional ex-pression分类
生物科学引用本文复制引用
王丹丹,刘晓牧,张冉,刘桂芬,万发春,林浴霜..鲁西黄牛MSTN基因上游序列多态性对转录表达的影响[J].中国畜牧兽医,2016,43(7):1667-1673,7.基金项目
山东省农业良种工程重大课题"优质肉牛新品系培育"和肉牛优异品质种质创新利用研究 （）
山东省攻关项目(2014GNC110028) （2014GNC110028）
