中国畜牧兽医2016，Vol.43Issue(7)：1702-1708,7.DOI:10.16431/j.cnki.1671-7236.2016.07.007
京海黄鸡中HOXC10基因表达规律及其生物信息学分析
Tissue Expression and Bioinformatics Analysis of HOXC10 Gene in Jinghai Yellow Chicken
摘要
Abstract
HOX genes express in nearly all eukaryotic cells such as yeast and human.To study tis-sue expression and bioinformatics of HOXC10 gene,the samples of heart,liver,spleen,lung,kid-ney,chest muscle,leg muscle,hypothalamus and ovary tissues were collected from 6 Jinghai Yellow chicken with 8-week-old to analyze the expression pattern of HOXC10 gene in different tissues by qRT-PCR method,and the different expression level were detected in ovary of 300 days old chickens in high yield group and low yield group.The bioinformatics of HOXC10 gene were analyzed by a variety of online softwares of Gallus gallus.The result showed that the expression level of HOXC10 gene in leg muscle was extremely significantly higher than that in other tissues of Jinghai Yellow chicken (P<0.01);And it’s expression level in low yield group was signifi-cantly higher than that in high yield group (P<0.05);HOXC10 gene of Gallus gallus shared 77.4%,94.8%,98.6%,68.3%,67.9%,66.0%,59.0%,68.1% and 79.4% with Meleagris gal-lopavo,Columba,Anas platyrhynchos,Homo sapiens,Mus musculus,Bos taurus,Sus scrofa, Capraaegagrus hircus and Xenopus laevis,respectively;HOXC10 gene encoded 354 amino acids in which serine was the most abundant;There were 24 potential phosphorylation sites and a HD in 280 to 342 position of amino acid sequence;The subcellular of the encoding protein was mainly located in the cell nucleus,and it had no cross membrane structure.HOXC10 gene might play an important role in regulation network of growth and reproduction in Jinghai Yellow chicken.关键词
HOXC1 0 基因/京海黄鸡/组织表达分析/生物信息学Key words
HOXC10 gene/Jinghai Yellow chicken/tissue expression analysis/bioinformatics分类
生物科学引用本文复制引用
张向前,董新龙,凌姣姣,韩昆鹏,张涛,王金玉,王永娟..京海黄鸡中HOXC10基因表达规律及其生物信息学分析[J].中国畜牧兽医,2016,43(7):1702-1708,7.基金项目
国家肉鸡产业技术体系(nycytx-42-G1-05) （nycytx-42-G1-05）
江苏高校优势学科建设工程 （）
江苏省动物遗传繁育与分子设计重点实验室 （）
