中国畜牧兽医2016，Vol.43Issue(7)：1709-1716,8.DOI:10.16431/j.cnki.1671-7236.2016.07.008
猪繁殖与呼吸综合征病毒强弱毒株全基因序列比对分析
Complete Genome Comparison and Analysis between Porcine Reproductive and Respiratory Syndrome Virus Parental and Its Attenuated Strains
摘要
Abstract
To study the attenuation molecular basis of porcine reproductive and respiratory syn-drome virus (PRRSV),whole genomes of PRRSV JXA1-5 (parental strain)and JXA1-86 (attenu-ated strain)were sequenced,compared and analyzed.And genomic sequences of JXA1/JXA1-86 were compared with the other two pairs of virulent parental/attenuated vaccine strains.The re-sults showed that there was higher amino acid mutation rate in structural proteins among the three pairs of strains indicating that PRRSV JXA1 strain virulence changes were more related to structure proteins variations.Compared with JXA1,the amino acids encoding Nsp5,Nsp6,Nsp8, Nsp12,M and N proteins of JXA1-86 strain did not change,suggesting that virulence attenuating might not be relevant to those proteins.The genomic sequences of each generation PRRSV se-quence were compared and analyzed,and the results showed that the variations among A928 V, I1155M,E1629D in ORF1a,I118V in GP2,H79N in GP3,I124V in GP4 and K59N in GP5 between 70th and 86th generations were found,which turned out that virulence attenuating might be associate with those variations.关键词
PRRSV JXA1 株/全基因组测序/毒力致弱Key words
PRRSV JXA1 strain/complete genome sequencing/virulence attenuating分类
农业科技引用本文复制引用
王业华,张欣欣,宋庆庆,李玉和,宫利娜,关平原..猪繁殖与呼吸综合征病毒强弱毒株全基因序列比对分析[J].中国畜牧兽医,2016,43(7):1709-1716,8.基金项目
农业部2015年动物疫情监测与防治项目 （）
