中国畜牧兽医2016，Vol.43Issue(7)：1723-1728,6.DOI:10.16431/j.cnki.1671-7236.2016.07.010
犬瘟热病毒V蛋白的表达、鉴定及亚细胞定位
Expression,Identification and Subcellular Localization of V Protein of Canine Distemper Virus
摘要
Abstract
In order to study the function of canine distemper virus (CDV)V protein,V gene frag-ment was inserted into the pGEX-6p-1 vector,and then pGEX-6p-1-CDV-V recombinant expres-sion plasmid was obtained.Purified V protein immunized BALB/c mice to prepare the positive se-rum.Meanwhile,to confirm the distribution of V protein and subcellular localization with confocal laser technology,a eukaryotic expression recombinant plasmid pcDNA3.1-CDV-V was built,then transfected into Vero cells.The results showed that V protein was successfully expressed and the positive serum was prepared.The recombinant plasmid pcDNA3.1-CDV-V was expressed in Vero cells and mainly expressed in cytoplasm.The results laid a function for functional studies of CDV V protein.关键词
犬瘟热病毒/V蛋白/原核细胞/亚细胞定位Key words
canine distemper virus/V protein/prokaryotic cell/subcellular localization分类
农业科技引用本文复制引用
刘莉,郝福星,袁维峰,封家旺..犬瘟热病毒V蛋白的表达、鉴定及亚细胞定位[J].中国畜牧兽医,2016,43(7):1723-1728,6.基金项目
公益性行业(农业)科研专项(201303042) （农业）
