中国畜牧兽医2016，Vol.43Issue(7)：1737-1742,6.DOI:10.16431/j.cnki.1671-7236.2016.07.012
贵州省猪源沙门氏菌对β-内酰胺类药耐药性及耐药基因分析
Analysis of Drug Resistance and Resistant Genes of Salmonella toβ-lactams Antimicrobial Agents Isolated from Pigs in Guizhou Province
摘要
Abstract
In order to analyse the resistance toβ-lactams antimicrobial agents and the prevalence of resistant genes of Salmonella in Guizhou province,130 Salmonella strains were isolated and iden-tified from 9 different regions of scale pig farms.The drug sensitivity to 8 kinds ofβ-lactams anti-microbial agents were determined by using the broth microdilution method.Allβ-lactams resistant isolates were detected for the presences of TEM,OXA,CTX-M and SHV genes by PCR.The re-sults showed that drug resistance of Salmonella to the commonly usedβ-lactams antimicrobial agents was very serious,and the resistance rate to ceftazidime was the highest (100%),followed by ampicillin and amoxicillin,were 76.15% and 80.77%,respectively.The resistance rates of ceft-iofur and cephalexin were the lowest (46.15%).Salmonella strains were all of multiple drug re-sistance,of which double resistance was at lowest (2.31%),and eightfold resistance was highest (4.62%),multidrug resistance mainly concentrated in fourfold to sevenfold,accounted for 88.46%.PCR results showed that TEM,OXA,CTX-M genes detection rate were 85%,75% and 46%,respectively,while the SHV gene was not inspected.Resistant phenotype was basically con-sistent with resistant genes.The results indicated that the resistance of Salmonella stains from pig toβ-lactams antimicrobial agents were widespread,and ceftazidime was particularly serious. The TEM,OXA and CTX-M genes were mainly carriedβ-lactams resistant genes in Salmonella isolates from Guizhou province.It had a great relationship between the prevalence of resistance genes and growth of antimicrobial resistance.关键词
沙门氏菌/β-内酰胺药物/药敏试验/耐药基因/耐药表型Key words
Salmonella/β-lactamas drugs/drug sensitivity test/resistance genes/resistant pheno-types分类
基础医学引用本文复制引用
曹正花,谭艾娟,吕世明,王雄,杜国琴..贵州省猪源沙门氏菌对β-内酰胺类药耐药性及耐药基因分析[J].中国畜牧兽医,2016,43(7):1737-1742,6.基金项目
贵州省科技厅农业攻关计划项目“生鲜乳安全保障关键技术集成与示范”(黔科合 NZ字[2012]3011号) （黔科合 NZ字[2012]3011号）
