中国畜牧兽医2016,Vol.43Issue(7):1780-1784,5.DOI:10.16431/j.cnki.1671-7236.2016.07.019
生地提取物对雏鸡免疫器官指数和H9疫苗免疫效果的影响
Effect of Radix rehmanniae Extract on Immune Organ Indexes and H9 Vaccine Immune Efficacy of Chicks
摘要
Abstract
This study was aimed to investigate the effect of the Radix rehmanniae extract on im-mune organs indexes and H9 vaccine immune efficacy.1 200 1-day-old healthy chicks fed with bas-al diet were chosen and randomly devided into 6 Groups on 14-day-old:GroupⅠ,Ⅱ and Ⅲ adding 2‰,3‰ 0.4‰Radix rehmanniae extract,respectively;Group Ⅳ adding 1‰ astragalus polysac-charide;Group Ⅴ immunned with H9 vaccine and fed with basal diet;Group Ⅵ was blank control group which didn’t immunned with H9 vaccine and fed basal diet.After 7 days medicine-feeding, the samples were collected at 14,21,28,35,42,49 and 56 days and the immune organs indexes and the H9 antibody levels were determined.The results showed as follows:①Compared with groupⅥ,the spleen and bursa indexes of groupⅠ,Ⅱ,Ⅲ and Ⅳ were significantly increased (P<0.05),indicating that dietary supplementation with Radixrehmanniae extract and astragalus pol-ysaccharide could improve spleen and bursa development (P<0.05).Compared with group ⅠandⅢ,the bursa and spleen indexes of group Ⅱ were significantly increased (P<0.05),while there was no significant difference between group Ⅱ and Ⅳ (P>0.05 ).②Dietary supplementation with Radix rehmanniae extract and astragalus polysaccharide could improve H9 antibody titers and hold it at a higher level.Compared with group Ⅰand Ⅲ,the H9 antibody titer of group Ⅱwas significantly increased from 42 days (P<0.05),while there was no significant difference be-tween group Ⅱ and Ⅳ (P>0.05).In conclusion,the Radix rehmanniae extract could improve immune organs development and immune function of chicks,and keep antibody titers at high lev-els when matches with H9 vaccine.And the recommended supplemental level of Radixrehmanni-ae extract was 2‰ in diet for chicks.关键词
雏鸡/地黄/黄芪多糖/免疫/H9 疫苗Key words
chicks/Radixrehmanniae/astragalus polysaccharide/immunization/H9 vaccine分类
农业科技引用本文复制引用
刘昭,陈立功,武现军,刘佳,郭莉..生地提取物对雏鸡免疫器官指数和H9疫苗免疫效果的影响[J].中国畜牧兽医,2016,43(7):1780-1784,5.基金项目
河北省现代农业产业技术体系蛋鸡产业创新团队专项资金资助 ()