中国畜牧兽医2016，Vol.43Issue(7)：1818-1825,8.DOI:10.16431/j.cnki.1671-7236.2016.07.025
环江香猪黏附素13基因多态性及其与仔猪腹泻的相关性分析
Association Analysis of MUC13 Gene Polymorphisms with Diarrhea in Huanj iang Miniature Pigs
摘要
Abstract
The purpose of the study was to analyze the association between MUC1 3 gene polymor-phisms and piglet diarrhea in Huanjiang miniature pigs.93 diarrhea piglets and 95 healthy controls were included in the study.The MUC1 3 gene polymorphisms were determined using high resolu-tion melting (HRM),and the results of HRM were verified by direct sequencing.Mutations were compared between diarrhea piglets and healthy controls.SHEsis online platform was used to cal-culate the haplotypes.There were statistically significant association on G224A site showed signif-icant difference between diarrhea piglets and healthy controls by allele frequencies (P<0.05),and there were also statistically significant differences between the two groups by genotypes among GG,GA,GG+GA and AA (P<0.05).But no statistically significant association was found be-tween polymorphisms of A112G,T170C and G164C sites and piglet diarrhea risk (P>0.05). However,haplotype Ⅹ (GTAC)in the distribution of diarrhea group was extremely significantly higher (P<0.01).The present data revealed that MUC13 gene G224A site and the haplotypes Ⅹ(GTAC)were associated with reduce risk of piglet diarrhea.关键词
环江香猪/黏附素 13 基因/单核苷酸多态性/单倍型/仔猪腹泻Key words
Huanjiang miniature pigs/MUC13 gene/SNP/haplotypes/piglet diarrhea分类
畜牧业引用本文复制引用
李俊,许钟峯,张海航,尚月丽,孔祥峰,石德顺,刘庆友..环江香猪黏附素13基因多态性及其与仔猪腹泻的相关性分析[J].中国畜牧兽医,2016,43(7):1818-1825,8.基金项目
国家高技术研究发展计划(863)项目(2013AA102504) （863）
国家自然基金项目(31260552) （31260552）
