中国畜牧兽医2016，Vol.43Issue(7)：1856-1861,6.DOI:10.16431/j.cnki.1671-7236.2016.07.030
1株鸽Ⅰ型副黏病毒的分离与鉴定
Isolation and Identification of One Strain of Pigeon Paramyxovirus SerotypeⅠ
摘要
Abstract
A pigeon paramyxovirus serotypeⅠ(PPMV-1)isolate was recovered from samples col-lected from dead pigeons and initial isolation of the virus was performed in 9-day-old specific-pathogen-free (SPF)chicken embryos.The isolate was identified by the hemagglutination (HA) test,hemagglutination-inhibition (HI)assays,RT-PCR,sequencing and analysis of partial of F gene of the virus.The virulence and pathogenicity of the isolate were determined by mean death time (MDT),intracerebral pathogenicity index (ICPI)in 1-day-old chicks and animal regression experiment.The results indicated that one PPMV-1 was isolated from dead pigeon and named as GXP120012.The F gene sequence analysis of PPMV-1 GXP120012 strain showed that the cleav-age sites of the virus was 112 R-R-Q-K-R-F117 motif in accordance with characteristic of velogenic strains;The nucleotide sequence homologies of F gene between strain GXP120012 and PPMV-1 reference strain pi/CH/LLN/110713 were high and reached 98.9%.They located the same branch on phylogenetic tree and fell into genotype Ⅵ.The MDT and ICPI values of isolate were 102 h and 0,respectively.The results of animal regression experiment showed that GXP120012 isolate was virulent for pigeons and avirulent for chickens.The present study provided useful material and theoretical basis for the study of molecular epidemiology and prevention and control of New-castle disease in pigeon in future.关键词
鸽Ⅰ型副黏病毒/分离鉴定/致病性/基因型Key words
pigeon paramyxovirus serotypeⅠ/isolation and identification/pathogenicity/genotype分类
农业科技引用本文复制引用
韦天超,翟国胜,李海琼,潘超,农海连,黄文飞,磨美兰,韦平..1株鸽Ⅰ型副黏病毒的分离与鉴定[J].中国畜牧兽医,2016,43(7):1856-1861,6.基金项目
国家自然科学基金项目(31360612) （31360612）
广西自然科学基金项目(2013GXNSFAA019080) （2013GXNSFAA019080）
广西科技攻关项目(桂科攻1123007-4) （桂科攻1123007-4）
广西大学生创新创业训练计划项目(201410593118、SYJN20121701) （201410593118、SYJN20121701）
