中国畜牧兽医2016，Vol.43Issue(7)：1862-1868,7.DOI:10.16431/j.cnki.1671-7236.2016.07.031
奶牛源MRSA菌株的分离鉴定和耐药性分析
Isolation,Indentification and Antibiotic Resistance Analysis of Methicillin-resistant Staphylococcus aureus (MRSA)from Raw Cow Milk
摘要
Abstract
In this study,we collected 502 california mastitis test (CMT)positive milk samples from several cattle farms and then the bacteria were isolated and identified from them.After that, we used multiplex-PCR for screening out methicillin-resistant Staphylococcus aureus (MRSA). Then we investigated the MRSA’s antibiotics susceptibility to 1 9 kinds of antibiotics by discdiffu-sion method.The results showed that 338 strains of Staphylococci including 89 strains of MRSA were detected out from the 452 milk samples with bacteria.Additionally,most MRSA showed high sensitivity to vancomycin,fosfomycin,chloramphenicol and clarithromycin,while resistant to penicillin,polymyxin,spectinomycin and ceftazidime.The results provided certain factual basis and method for guiding the use of antibiotics in bovine mastitis,rapid identification of MRSA strains and prevention or control of MRSA infection.关键词
奶牛乳房炎/耐甲氧西林金黄色葡萄球菌/多重PCR/药敏Key words
bovine mastitis/MRSA/multiplex-PCR/drug sensitivity分类
农业科技引用本文复制引用
冯清,秦爱建,金文杰,林伟东,张大帅,戴迪,廖俊,王仙,汪怿旸,梁祥焕,张军..奶牛源MRSA菌株的分离鉴定和耐药性分析[J].中国畜牧兽医,2016,43(7):1862-1868,7.基金项目
江苏省“青蓝工程” （）
扬州市社会发展科技攻关项目(YZ2011091) （YZ2011091）
江苏省农业科技自主创新资金项目(CX(14)2088) （CX(14）
江苏高校优势学科建设工程(PAPD) （PAPD）
常州市科技支撑项目(CE20152006) （CE20152006）
