中国畜牧兽医2016，Vol.43Issue(7)：1869-1875,7.DOI:10.16431/j.cnki.1671-7236.2016.07.032
葫芦多糖体外抗鸡新城疫病毒作用的研究
Study on Antiviral Effect of Polysaccharides from Lagenaria siceraria (Molina)Standl.on NDV in vitro
摘要
Abstract
To research on polysaccharides from Lagenaria siceraria (Molina ) Standl.against Newcastle disease virus (NDV).Total polysaccharide and four different solvent extractions of Lagenaria siceraria (Molina)Standl.were extracted by the methods of hot water extraction and ethanol precipition in this study.The content of polysaccharide was measured by phenol-sulfuric acid method and infrared spectroscopy method.The safe concentration and growth of chick em-bryo fibroblast (CEF)were assayed by MTT method,in order to facilitated the comparison under the same level,the safe concentration was united as 78.125μg/mL.Under the safety range of con-centration,detected the block-virus activity,anti-virus activity and virus-killing activity of poly-saccharides through the ways of pre-adding polysaccharides,post-adding polysaccharides and adding polysaccharides with NDV.The results showed that the direct inactivation and propagation inhibition activity of total polysaccharide and four different solvent extractions were stronger than anti-absorption function.Anti-virus inhibition rate of 70%,80% gradient alcohol precipitation of polysaccharides from Lagenaria siceraria (Molina)Standl.(LSP70 ,LSP80 )and total polysaccha-ride (LSPt )were 40.41%,44.54% and 61.85%,virus-killing inhibition rate were 44.74%, 58.76% and 59.38%.LSP80 had the highest virus inhibition rate as 37.14% in the block-virus ac-tivity of those five polysaccharides.In summary,70%,80% gradient alcohol precipitation and total polysaccharide in polysaccharides possessed better activity and would be as the materials for fur-ther research.关键词
葫芦/多糖/鸡胚成纤维细胞/新城疫病毒Key words
Lagenaria siceraria (Molina)Standl./polysaccharide/chick embryo fibroblast/New-castle disease virus分类
畜牧业引用本文复制引用
阿得力江·吾斯曼,德力拜尔,努尔艾力·麦提尼亚孜,布合丽倩穆·依明,赛福丁·阿不拉..葫芦多糖体外抗鸡新城疫病毒作用的研究[J].中国畜牧兽医,2016,43(7):1869-1875,7.基金项目
国家自然科学基金(31360625) （31360625）
