中国药房2016，Vol.27Issue(20)：2768-2770,3.DOI:10.6039/j.issn.1001-0408.2016.20.10
UPLC-MS/MS法同时测定患儿血浆中乙胺丁醇及异烟肼的浓度
Simultaneous Determination of Ethambutol and Isoniazid in Children’s Plasma by UPLC-MS/MS
摘要
Abstract
OBJECTIVE:To establish a method for the simultaneous determination of ethambutol(EMB)and isoniazid(INH) in children’s plasma. METHODS:After precipitated with acetonitrile,plasma samples were determined by UPLC-MS/MS method. Using lamivudine as internal standard,Waters ACQUITY® UPLC HSS T3 column was adopted with mobile phase consisted of ace-tonitrile-water(containing 0.05% formic acid and 15 mmol/L ammonium formate)in gradient mode at the flow rate of 0.2 ml/min. By ESI,positive ion detection was conducted in MRM mode. The monitoring transition ion-pair was m/z 205.2→116.0 for EMB, m/z 138.1→121.1 for INH and m/z 230.2→111.9 for internal standard. RESULTS:The linear range of EMB and INH was 10-5 000 ng/ml and 50-5 000 ng/ml,respectively;the limits of quantitation were 10 and 50 ng/ml;RSDs of inter-day and intra-day were all lower than 15% and relative recoveries were 98.7%-105.5%. Plasma concentrations of EMB and INH in 4 children with tuberculo-sis were 94.5-99.7 and 55.1-80.9 ng/ml. CONCLUSIONS:The method is rapid and sensitive,which suitable for children’s plasma concentration monitoring of EMB and INH and pharmacokinetic study.关键词
乙胺丁醇/异烟肼/超高效液相色谱-串联质谱法/血药浓度/肺结核/患儿Key words
Ethambutol/Isoniazid/UPLC-MS/MS/Plasma concentration/Pulmonary tuberculosis/Children分类
医药卫生引用本文复制引用
安静,董占军,张笑丹,刘洪涛..UPLC-MS/MS法同时测定患儿血浆中乙胺丁醇及异烟肼的浓度[J].中国药房,2016,27(20):2768-2770,3.基金项目
河北省医学科学研究重点课题计划项目 （）
