摘要
Abstract
OBJECTIVE:To establish a method for the content determination of berberine hydrochloride in Fufang huangge ji-angtang tablet. METHODS:UPLC was performed on the column of Acquity UPLC BEH C18 with mobile phase of acetoni-trile-0.1% H3PO4(21∶79,V/V)at flow rate of 0.3 ml/min,the detection wavelength was 345 nm,column temperature was 30℃, and the volume injection was 1.0 µl. RESULTS:The linear range of berberine hydrochloride was 0.008 16-0.081 6 µg(r=0.999 9);RSDs of precision,stability and reproducibility were lower than 1%;average recovery was 97.05%-98.96%(RSD=0.66%,n=6). CONCLUSIONS: The method is simple,precise,stablity and reproducible,and can be used for the quality control of Fufang huangge jiangtang tablet.关键词
复方黄葛降糖片/盐酸小檗碱/超高效液相色谱法Key words
Fufang huangge jiangtang tablet/Berberine hydrochloride/UPLC分类
医药卫生
