中国医科大学学报2016，Vol.45Issue(7)：577-582,6.DOI:10.12007/j.issn.0258-4646.2016.07.001
ING5基因表达对胃癌细胞生长抑制作用的研究
Anti-tumor Effects of ING5 Gene on Gastric Cancer
摘要
Abstract
Objective To study the effect of ING5 gene on growth inhibition of gastric cancer. Methods ING5 expressing plasmid was transfect⁃ed into SGC⁃7901 cells. The cell viability was assessed by CCK⁃8,cell apoptosis and cycle was detected by flow cytometry analysis,the migration and invasion was evaluated by scratch and transwell,the expressions of mRNA and protein were determined by real⁃time quantitative PCR and West⁃ern blot respectively. Nude mice were implanted subcutaneously with SGC⁃7901 cells and tumor size and related protein were analyzed. Results After transfection of pEGFP⁃N1⁃ING5,the proliferation of gastric cancer SGC⁃7901 cells was significantly inhibited(P<0.05),the apoptosis was decreased(P<0.05),the percentage of G1 phase was increased and G2 phase was decreased(P<0.05),the ability of migration and invasion was reduced(P<0.05),the expression of NF⁃κB,PI3K,p⁃Akt1/2/3,Bcl⁃2,XIAP,β⁃catenin,c⁃myc mRNA,and protein levels were significantly in⁃creased(P<0.05),and the expression of Akt1/2/3、MMP9 mRNA and protein levels were significantly decreased(P<0.05). Conclusion The ING5gene inhibits the SGC⁃7901 cell proliferation,induces G1 arrest,inhibits the migration and invasion,and effectively regulates the related genes and proteins about cell proliferation,cell cycle and adhesion,but reduces apoptosis. ING5 can inhibit the evolution and development of gastric can⁃cer.关键词
胃癌/ING5/肿瘤发生/基因治疗Key words
gastric cancer/ING5/tumorgenesis/gene therapy分类
临床医学引用本文复制引用
杨雪峰,勾文峰,赵爽,申道福,吴亚州,李君君,郑华川..ING5基因表达对胃癌细胞生长抑制作用的研究[J].中国医科大学学报,2016,45(7):577-582,6.基金项目
国家自然科学基金（81172371；81472544）；辽宁省教育厅科学研究基金（LJQ2014093）；锦州医科大学附属第一医院校长基金 （）
