中国医科大学学报2016，Vol.45Issue(7)：583-586,4.DOI:10.12007/j.issn.0258-4646.2016.07.002
miR-21对HeLa细胞增殖、凋亡和MMP2基因表达的影响
Effects of miR-21 on the Proliferation,Apoptosis and MMP2 Expression of HeLa Cells
摘要
Abstract
Objective To study the effects of miR⁃21 on the proliferation,apoptosis and MMP2 expression of HeLa cells. Methods The miR⁃21 or the miRNA scramble control was transfected into Hela cells. The cell proliferation was detected by the Celltiter⁃GloTM assay 72 h after the transfection,and the apoptosis was evaluated by the Caspase3/7 Glo® Kit. The MMP2 RNA expression was quantified by quantitative real time PCR. Results The proliferation of HeLa cells transfected with miR⁃21 was significantly increased compared to that of the cells transfected with miRNA scramble control. The caspase 3/7 activity in HeLa cells transfected with miR⁃21 was downregulated compared to that of the cells transfect⁃ed with miRNA negative control. The MMP2 RNA expression in HeLa cells transfected with miR⁃21 was increased significantly compared to the cells transfected with miRNA negative control. Conclusion miR⁃21 can significantly promote the proliferation of HeLa cells. In addition,it ex⁃hibits the anti⁃apoptosis effect in HeLa cells. The transfection of miR⁃21 significantly increased the MMP2 RNA expression,which suggests that miR⁃21 may promote the tumor invasion and might be a therapeutic intervention target.关键词
miR-21抑制剂/转染/增殖Key words
miR-21/transfection/proliferation分类
临床医学引用本文复制引用
郭琳琳,李哲,柯娴,陈志伟,吴南锦,张越..miR-21对HeLa细胞增殖、凋亡和MMP2基因表达的影响[J].中国医科大学学报,2016,45(7):583-586,4.基金项目
国家自然科学基金面上项目（81070128）；教育部留学启动基金（20121707）；人力资源与社会保障部科技项目择优资助（2015）；辽宁省教育厅优秀人才项目（L2011112）；沈阳医学院优秀人才项目（20113067）；大学生科研立项课题 （）
