中国医科大学学报2016，Vol.45Issue(7)：587-590,4.DOI:10.12007/j.issn.0258-4646.2016.07.003
环磷酰胺腹腔注射法及膀胱灌注法制备膀胱过度活动大鼠模型的研究
Construction of Experimental Animal Model of Overactive Bladder in Rat Induced by Cyclophosphamide Intraperitoneal Injection and Intravesical Instillation
摘要
Abstract
Objective To compare the advantages and disadvantages of two methods for rat overactive bladder(OAB)model construction in⁃duced by intraperitoneal injection and intravesical instillation cyclophosphamide. Methods A total of 30 female SD rats weighting 200⁃250 g were randomly categorized into three groups:intraperitoneal injection⁃induced OAB(Ip⁃OAB),intravesical instillation⁃induced group(Iv⁃OAB)and con⁃trol group. Ip⁃OAB rats was i.p. administrated cycbophosphe mide three times in dose of 75 mg/kg body weight,while Iv⁃OAB rats received intravesi⁃cal instillation three times in drug dose of 75 mg/kg body weight. Control group rats received no treatment. Maximum bladder capacity(MBC),maxi⁃mum voiding pressure(MVP),frequency of spontaneous contraction of each group were recorded. The incidence,the mortality and the pathology of the three groups were compared. Results MBC,MVP and frequency of spontaneous contraction between Ip⁃OAB group and Iv⁃OAB group had no statistically significant difference(P>0.05). Compared with the control group,MBC significantly increased(P<0.05),MVP significantly decreased (P<0.05),and frequency of spontaneous contraction significantly increased(P<0.05)in Ip⁃OAB and Iv⁃OAB rats. The modeling success rate and mortality were 100%and 80%in Ip⁃OAB group,and were 50%and 0%in Iv⁃OAB group,and pathological changes were found in the two groups. Conclusion The construction of experimental animal model of OAB in rat induced by intraperitoneal injection and intravesical instillation cyclo⁃phosphamide are both reliable methods. Ip⁃OAB rats exhibit high incidence and mortality rate,while Iv⁃OAB rats show low incidence and mortality rate.关键词
膀胱过度活动/膀胱灌注/腹腔注射/不同方法Key words
overactive bladder/intraperitoneal injection/intravesical instillation/different methods分类
医药卫生引用本文复制引用
段乙南,夏志军,许海楠..环磷酰胺腹腔注射法及膀胱灌注法制备膀胱过度活动大鼠模型的研究[J].中国医科大学学报,2016,45(7):587-590,4.基金项目
国家科技部十二五“重大新药创制”科技重大专项课题（2012ZX09401004）；辽宁省自然科学基金（20102255）；辽宁省科学技术计划 （）
