中国医科大学学报2016，Vol.45Issue(7)：591-595,600,6.DOI:10.12007/j.issn.0258-4646.2016.07.004
SAHA和TRAIL联合使用对三阴性乳腺癌细胞MDA-MB-231生长状态的影响
Effects of Combination of SAHA and TRAIL on Proliferation of Triple-negative Breast Cancer Cell MDA-MB-231
摘要
Abstract
Objective To investigate the effects of combined treatment of suberoylanilide hydroxamic acid(SAHA)and TNF⁃related apoptosis inducing ligand(TRAIL)on proliferation and morphology change of triple⁃negative breast cancer cell MDA⁃MB⁃231. Methods The effects of combination treatment of SAHA and TRAIL on proliferation and morphology change of MDA⁃MB⁃231 cells were monitored by RTCA. Morphology changes of MDA⁃MB⁃231 cells by different treatment factors were observed through time⁃lapse live cell imaging acquisition. Results Real⁃time cell proliferation assays showed that a synergistic effect were found when MDA⁃MB⁃231 cells were treated with combination of SAHA and TRAIL , and reached the best effect with 5μmol/L SAHA and 50 ng/mL TRAIL. The results of time⁃lapse live cell imaging acquisition showed that the growth inhibition of MDA⁃MB⁃231 cells with combined treatment of SAHA and TRAIL were more obvious than that with treatment of SAHA or TRAIL alone. Conclusion The combined treatment of SAHA and TRAIL induces a synergistic effect on growth inhibition in triple⁃negative breast cancer cell line MDA⁃MB⁃231.关键词
辛二酰苯胺异羟肟酸/肿瘤坏死因子相关凋亡诱导配体/三阴性乳腺癌细胞Key words
suberoylanilide hydroxamic acid/TNF-related apoptosis inducing ligand/triple-negative breast cancer cell分类
基础医学引用本文复制引用
韩翰,徐佳..SAHA和TRAIL联合使用对三阴性乳腺癌细胞MDA-MB-231生长状态的影响[J].中国医科大学学报,2016,45(7):591-595,600,6.基金项目
国家自然科学基金（81172509）；辽宁省教育厅科学研究一般项目 （）
