中国医科大学学报2016，Vol.45Issue(7)：610-615,6.DOI:10.12007/j.issn.0258-4646.2016.07.008
瞬时受体电位通道蛋白的跨膜片段分析
Transmembrane Segment Analysis of Transient Receptor Potential Channel
摘要
Abstract
Objective To investigate the membrane topology of transient receptor potential(TRP)channel. Methods Glycosylation method was used to investigate the membrane integrations of each hydrophobic segment of canonical TRP(TRPC5). Results In TRPC5 channel,S4⁃S8 segments were integrated into membrane with Ncyt/Cexo and Nexo/Ccyt orientations sequentially ,and C⁃terminus was intracellular. S1⁃S3 segments were integrated into membrane with two possible types. One was that S1 and S3 were integrated into membrane,whereas S2 was left out of the membrane on the cytosolic side;and the other was a mixed type that S1 and S3 were exposed to cytoplasm respectively. Both of them,the N⁃termi⁃nus was intracellular. Conclusion S4⁃S8 segments of TRPC5 are transmembrane segments. The integrations of S1⁃S3 segments into membrane need to be further investigated.关键词
瞬时受体电位通道/膜拓扑结构/跨膜片段分析Key words
transient receptor potential channel/membrane topology/transmembrane segment analysis分类
基础医学引用本文复制引用
苏秋香,张丽艳,汲坤,张改改,张利华..瞬时受体电位通道蛋白的跨膜片段分析[J].中国医科大学学报,2016,45(7):610-615,6.基金项目
辽宁省科技厅科学事业公益研究基金（2014001024）；沈阳医学院科学研究基金 （）
