中国医科大学学报2016，Vol.45Issue(8)：719-722,727,5.DOI:10.12007/j.issn.0258-4646.2016.08.012
胶质瘤中STAT3通路及其负调控因子PIAS3活化状态和生物学效应的分析
Activation Status and Biological Effects Analysis of STAT3 Signaling Pathway and Its Negative Regulator PIAS3 in Gliomas
摘要
Abstract
Objective To discuss the activation and biological effects of STAT3 signaling pathway and its negative regulator PIAS3 in glioma cells. Methods Immunohistochemistry(IHC)was used to test the expression of p⁃STAT3 and PIAS3 in gliomas. AG490(60μmol/L),the in⁃hibitor of STAT3 signaling pathway,was used to treat U118 and U87 cells for 24/48 h,and the method of MTT assay was taken to evaluate the pro⁃liferation after AG490 treatment. The expression of p⁃STAT3 and PIAS3 was also examined by immunofluorescence(IF). Results There was no obvious significance between p⁃STAT3 and PIAS3 in nuclei or cytoplasm at different grades of gliomas. Whereas,p⁃STAT3 and PIAS3 were nega⁃tively correlated in the nuclei of vary grades malignancy gliomas. After AG490 treatment,U118 cells showed no obvious quantitative changes. How⁃ever,U87 cells showed obvious growth inhibition. IF results showed that there was no significant change at the levels of p⁃STAT3 and PIAS3 after AG490 treatment in U118 cells. However,the expression of p⁃STAT3 in the nuclei was down⁃regulated,and PIAS3 showed obvious nuclear translo⁃cation in U87 cells. Conclusion Nuclear translocation of PIAS3 plays the key role in modulating JAK/STAT signaling activation and inhibiting glioma cells proliferation.关键词
胶质瘤/STAT3信号通路/PIAS3/AG490Key words
glioma/STAT3 signaling pathway/PIAS3/AG490分类
临床医学引用本文复制引用
张朋,孙铮,刘哲宇,李阳..胶质瘤中STAT3通路及其负调控因子PIAS3活化状态和生物学效应的分析[J].中国医科大学学报,2016,45(8):719-722,727,5.基金项目
中国博士后科学基金面上项目 （）
