中国药理学通报2016，Vol.32Issue(7)：950-955,6.DOI:10.3969/j.issn.1001-1978.2016.07.013
趋化因子 MCP-1对大鼠海马区NMDA受体介导的兴奋性突触后电流的影响
Effects of chemokine MCP-1 on NMDA-mediated exciatory postsynaptic current in hippocampal slice of rats
摘要
Abstract
Aim To explore how MCP-1 induces neu-rodisorder by determing the effects of MCP-1 on excita-tory postsynaptic current(EPSCs) in the CA1 region of rat hippocampal brain slices .Methods EPSCs, the AMPA receptor-mediated EPSC (EPSCAMPAR ), NMDA receptor mediated EPSCs(EPSCNMDAR) and NR2BR re-ceptor-mediated EPSC ( EPSCNR2BR ) were recorded u-sing whole-cell patch recording techniques to observe the effects of 2.3 nmol· L-1 MCP-1 on pyramidal neu-rons in hippocampal CA1 region.Microtubule-associat-ed protein-2 ( MAP-2 ) staining was used to study whether MCP-1 induced dendritic injuries in hippocam-pal CA1 region and whether NMDAR , AMPAR or CCR2 receptor antagonists had protective effects a-gainst dendritic damage caused by MCP-1.Results ① Bath application of MCP-1 produced a significant enhancement of the amplitudes of EPSCs , EPSCAMPAR and EPSCNMDAR .②Further studies revealed that MCP-1 potentiated EPSC NR2BR; ③ The MCP-1-associated dendritic injuries were blocked by NMDAR , AMPAR and CCR2R antagonists respectively .Conclusions Our results suggest a potential role of MCP-1 which may play in neuroexcitotoxicity and neural injury via NMDA receptor(especially NMDAR subtype NR2BR) and CCR2 receptor .The antagonists of these receptors may have potential therapeutic effect for neurodegener-ation.关键词
趋化因子/MCP-1/海马脑片/兴奋性突触后电流/神经兴奋性毒性/艾滋性认知功能障碍Key words
chemokine/MCP-1/hippocampal slices/EPSCs/neuroexcitoxicity/HAND分类
医药卫生引用本文复制引用
李珊,胡喆,周燕,熊焕贵..趋化因子 MCP-1对大鼠海马区NMDA受体介导的兴奋性突触后电流的影响[J].中国药理学通报,2016,32(7):950-955,6.基金项目
国家自然科学基金课题（ No 81360192）；广西自然科学基金（No 2012GXNSFCA053004）；广西教育厅高等学校资助科研项目 （）
