中国药理学通报2016，Vol.32Issue(7)：966-969,970,5.DOI:10.3969/j.issn.1001-1978.2016.07.016
水飞蓟素对人骨肉瘤 Saos-2细胞的抑制作用及相关机制
Inhibitory effect of silymarin on human osteosarcoma Saos-2 cells and its mechanism
摘要
Abstract
Aim To study the effect of silymarin on proliferation and apoptosis of human osteosarcoma saos-2 cells and to explore its possible mechanisms .Meth-ods Control group and different concentration groups of silymarin were set , and Saos-2 cells were treated with silymarin .Celluar morphologic changes were ob-served by inverted phase contrast microscope .Prolifer-ation of cells was tested CCK-8 assay .Apoptosis rate of cells was analyzed by flow cytometry . ERK1/2、p-ERK1/2 and cleaved caspase-3 protein expression were measured by Western blot .Results Silymarin inhibi-ted the proliferation of Saos-2 cells in a time-dependent and concentration-dependent manner . Silymarin in-duced the apoptosis of Saos-2 cells in a concentration-dependent manner . Silymarin decreased p-ERK1/2 protein expression and increased cleaved caspase-3 protein expression in a concentration-dependent man-ner, while ERK1/2 protein expression had no obvious change.Conculsions Silymarin can inhibit the prolif-eration of human osteosarcoma saos-2 cells and induce apoptosis of them , and the mechanism may be related to inhibition of ERK signaling pathway and upregulated caspase-3 protein expression .关键词
水飞蓟素/骨肉瘤/增殖/凋亡/机制/ERK 信号通路/caspase-3Key words
silymarin/osteosarcoma/proliferation/apoptosis/mechanism/ERK signaling pathway/caspase-3分类
医药卫生引用本文复制引用
严晨,邬亚华,王子瑶,殷嫦嫦,殷明..水飞蓟素对人骨肉瘤 Saos-2细胞的抑制作用及相关机制[J].中国药理学通报,2016,32(7):966-969,970,5.基金项目
国家自然科学基金资助项目 （）
