中国药理学通报2016，Vol.32Issue(7)：991-997,7.DOI:10.3969/j.issn.1001-1978.2016.07.021
益生菌对大鼠酒精性肝损伤的保护作用及机制研究
Effects of probiotics on alcoholic liver injury in rats and its mechanisms
摘要
Abstract
Aim To observe the protective effects of probiotics on alcoholic liver injury in rats .Methods Male Wistar rats were randomly divided into the follow-ing three groups: control group , normal diet with nor-mal (5 ×108 CFU· kg -1· d -1) treatment group.Ex-cluding the rats in the normal control group , the other animals were initially received intragastric administra-tion with 56%( V/V) ethanol 5.5~11.0 mL· kg -1 · day -1 for 8 weeks.Then the rats’ faeces were collect-ed, and the liver and the small intestine were obtained for pathologic and ultrastructural observation .Serum ALT, AST and ALP was measured by method of bio-chemistry .Serum DAO and D-LA was measured by en-zyme linked immunosorbent assay .The expression of FOXO4 in small intestine was detected by immunohis-tochemistry .The intestinal flora genome DNA was ex-tracted from faeces and the sequence of 16 S rDNA was analyzed by high-throughput sequencing technologies . Results Hepatic steatosis was obviously improved in probiotics treatment groups compared with ethanol-trea-ted group , and the ultrastructural such as mitochondri-al and rough endoplasmic reticulum pathological chan-ges was significantly alleviated . The ultrastructural changes in intestinal were better in probiotics treatment group than in the ethanol-treated group .And ethanol-induced rats ’ serum ALT, AST, ALP, D-LA and DAO levels showed a significant reduction in the probi-otics treatment groups compared with the ethanol-trea-ted group ( P<0.05 ) .The FOXO4 expression was in-creased obviously in the probiotics treatment groups compared with the ethanol-treated group ( P <0.05 ) . And the intestinal flora diversity was impacted after feeding alcohol , and probiotics had a certain regulative action in helping the intestinal flora back to normal state; At phylum level , the Firmicutes quantity was lower and the Bacteroidetes quantity was higher in eth-anol-treated group than those in the control group ( P<0.05 ) , and the conditions were improved after supple-menting probiotics .At genus level , the percent of ge-nus abundance was similar to normal control group in the probiotics treatment groups compared with the etha-nol-treated group .Conclusion Probiotics can relieve liver injury induced by alcohol in rats , and the mecha-nism may be related to the modulation of probiotics on the intestinal flora distribution and intestinal barrier .关键词
益生菌/酒精性肝损伤/肠道屏障功能/肠道菌群/16 S rDNA高通量测序/分子生态学Key words
probiotics/alcoholic liver disease/intesti-nal barrier/intestinal flora/16 s rDNA high throughput sequencing/molecular ecology分类
医药卫生引用本文复制引用
梁惠,吕锐,傅泳,周治彤,刘颖,周晓彬,王文成,刘曼,马爱国..益生菌对大鼠酒精性肝损伤的保护作用及机制研究[J].中国药理学通报,2016,32(7):991-997,7.基金项目
国家自然科学基金资助项目（ No 81573137）；达能营养中心膳食营养研究与宣教基金资助项目（ No DIC2014-03）；青岛大学创新型教学实验室研究项目 （）
