中国药理学通报2016，Vol.32Issue(7)：1012-1016,5.DOI:10.3969/j.issn.1001-1978.2016.07.024
灵芝多糖联合二甲双胍对2型糖尿病大鼠心肌结构及血流动力学的影响
Effects of Ganoderma lucidum polysaccharides combined with metformin on myocardial structure and hemodynamics in type 2 diabetic rats
摘要
Abstract
Aim To discuss the effects and mechanism of Ganoderma lucidum polysaccharides and metformin on myocardial structure and hemodynamics in type 2 diabetic rats.Methods High fat diet combined with intraperitoneal injection of low dose streptozotocin 30 mg· kg -1 was applied to establish rat model of type 2 diabetes mellitus .The diabetic rats were randomly into normal control group ,diabetes group , ganoderma lucid-um polysaccharides group (600 mg· kg -1 ) , metformin group ( 600 mg · kg -1 ) , combination group ( ganoder-ma lucidum polysaccharides 300 mg · kg -1 +metform-in 300 mg· kg -1 ) .After 12 weeks′treatment,the lev-els of fasting serum glucose were determined and the hemodynamic parameters (LVSP,LVEDP,dp/dtmax,-dp/dtmax ) were determined.Collagen volume fraction ( CVF ) was detected by Van Gieson . Immunohisto-chemical method and Western blot were used to detect myocardial tissue MMP-2 protein expression .Results The fasting blood glucose was significantly decreased in the combined treatment group .Combined medication could significantly improve hemodynamic parameters in diabetic rats: reduced LVEP and raised LVEDP , dp/dtmax and -dp/dtmax .CVF was significantly decreased in combination group .The expression of MMP-2 in my-ocardial tissue was significantly inhibited .Conclusions The combination of Ganoderma lucidum polysaccha-ride and metformin can significantly improve the hemo-dynamic parameters in type 2 diabetic rats, and have a preventive effect on diabetic cardiomyopathy . The mechanism may be related to the down regulation of the expression of MMP-2.关键词
灵芝多糖/二甲双胍/糖尿病大鼠/心肌结构/血流动力学/基质金属蛋白酶-2Key words
Ganoderma lucidum polyccharide/met-formin/diabetes mellitus rats/myocardial structure/hemodynamics/matrix metalloproteinase-2引用本文复制引用
乔进,窦志华,吴锋,孟国梁,陈惠,郑惠华..灵芝多糖联合二甲双胍对2型糖尿病大鼠心肌结构及血流动力学的影响[J].中国药理学通报,2016,32(7):1012-1016,5.基金项目
科技部十一五国家科技支撑计划项目（ No 2006DAI06A20-02）；江苏省优势学科项目 （）
