中国药理学与毒理学杂志2016，Vol.30Issue(7)：736-740,5.DOI:10.3867/j.issn.1000-3002.2016.07.005
苦参碱和氧化苦参碱致小鼠肝毒性比较
Comparison of liver toxicity of matrine and oxymatrine in m ice
摘要
Abstract
OBJECTIVE To compare the liver toxicity of matrine and oxymatrine ,and to explore their toxic mechanism. METHODS Thirty ICR mice were randomly divided into normal control ,matrine 200 mg · kg-1 and oxymatrine 200 mg · kg-1 groups,10 mice per group. After single ig administration of corresponding drugs or water, animal mortality was calculated at the 15th day. The content of glutamic-pyruvic transami⁃nase(GPT),glutamic-oxalacetic transamin(GOT),alkaline phosphatase(ALP)and lactate dehydroge⁃nase (LDH) in serum were detected. Histopathological changes of the liver were examined by HE stain. The content of superoxide dismutase(SOD)and glutathione(GSH)in liver homogenates were detected by ELISA. Hepatocyte apoptosis was detected by Tunel stain. RESULTS The mortality rate of mice in two groups was 80% and 0,respectively. GPT,GOT and ALP contents of dead mice in matrine group were significantly higher than that in normal control group(P<0.05). In oxymatrine group,only the content of ALP was increased(P<0.05). Four of the eight dead mice in matrine group exhibited liver cell necrosis(P<0.05),while only 1/10 mice in oxymatrine group had a mild liver cell necrosis(P>0.05). The content of SOD and GSH of dead mice in matrine group was lower than that in control group(P<0.05,P<0.01). The content of GSH in oxymatrine group was also decreased(P<0.05). The apoptosis rate of liver cells in dead mice in matrine group was increased(P<0.05). CONCLUSION A large dose of matrine and oxymatrine can produce liver toxicity. At an equal dosage,the liver toxicity of matrine is significantly higher than that of oxymatrine. The toxic mechanism is related to oxidative stress and apoptosis.关键词
苦参碱/氧化苦参碱/肝毒性Key words
matrine/oxymatrine/hepatotoxicity分类
医药卫生引用本文复制引用
郭秋平,金若敏..苦参碱和氧化苦参碱致小鼠肝毒性比较[J].中国药理学与毒理学杂志,2016,30(7):736-740,5.基金项目
国家重点基础研究发展计划（2009CB522807），广州市生物产业重大专项（201300000053）；广州市海珠区科技计划项目（2012-CG-01）@@@@The project supported by National Key Basic Research and Development Program（2009CB522807）；Major Bioscience and Technology Project of Guangzhou City（201300000053）；and Science and Technology Project of Haizhu District in Guangzhou City （）
