中国医学创新2016，Vol.13Issue(19)：5-9,5.DOI:10.3969/j.issn.1674-4985.2016.19.002
PM2.5对儿童骨髓基质细胞增殖及细胞因子G-CSF、GM-CSF分泌的影响
Effects of PM2.5 on the Proliferation and Secretion Levels of G-CSF and GM-CSF in Children’s BMSCs
摘要
Abstract
Objective:To observe the effects of ambient particulate matter (PM2.5) on the proliferation and secretion levels of cytokines of children’s bone marrow stromal cells (BMSCs) and to explore the effects of PM2.5 on children’s bone marrow hematopoietic microenvironment.Method:The collected BMSCs were exposed to different concentration of PM2.5 solutions.The proliferation of BMSCs and the secretion levels of G-CSF, GM-CSF and mRNA were detected.Result:The BMSCs proliferation increased with the increase of concentration of PM2.5 solution,after 50μg/mL the proliferation of BMSCs level gradually decline.The proliferation of BMSCs level with concentration 20μg/mL of experimental group was significantly higher than control,while 100μg/mL of experimental group was significantly lower than control group,the differences were statistically significant (P<0.05).The low concentration of PM2.5 with 10 and 20μg/mL promoted the secretion levels of G-CSF, GM-CSF and mRNA,while gradually inhibited in the PM2.5 concentration of 50,100 and 200μg/mL.The concentration reaches more than 100μg/mL,inhibiting effects reached significant level.Conclusion:The high concentration of PM2.5 has a certain toxic effect on children’s BMSCs.关键词
大气细颗粒物/骨髓基质细胞/增殖/细胞因子Key words
Ambient particulate matter/Bone marrow stromal cells/Proliferation/Cytokines引用本文复制引用
阳静,陈丽琼,叶中绿,刘丽丽..PM2.5对儿童骨髓基质细胞增殖及细胞因子G-CSF、GM-CSF分泌的影响[J].中国医学创新,2016,13(19):5-9,5.基金项目
广东省科技计划项目（40101S010004）；广东湛江市重点科技专项 （）
