中国医学创新2016，Vol.13Issue(20)：27-32,6.DOI:10.3969/j.issn.1674-4985.2016.20.007
异种源性脱细胞神经支架修复坐骨神经损伤后再生的可行性
The Feasibility of Xenogeneic Acellular Nerve Scaffold to Repair Rat Sciatic Nerve Defect Regeneration
摘要
Abstract
Objective:To evaluate remodeling effect of xenogeneic acellular nerve scaffold in a rat sciatic nerve defect model.Method:Scaffolds derived from rabbit tibial nerves were processed by extractive method,and results were monitored by HE,toluidine blue,immunohistochemistry, SEM,TEM.SD rats were randomly divided into two groups:xenogeneic acellular nerve scaffold group(experimental group) and autograft (control group) group.During 12 weeks postoperatively,the amount of CD3+,CD4+,CD8+ in blood were measured to evaluate the systematic immunologic function.Regenerative results were determined by SFI test,and histological examination. Result:Cell component and myelin sheath were completely removed and intact basal lumina tubes were persistent. During observation,all animals had no sign of immunological rejection systematically,grafts bridged well without any disintegration.In the experimental group,the amount of CD3+,CD4+,CD8+ in blood were not different with the control groups at 1,4,12 weeks postoperatively(P>0.05).Statistical analysis revealed that experimental group had no statistically significant differences between the experimental group and control group on aspect of SFI at 4,12 weeks(P>0.05).There had no statistically significant differences between the experimental group and control group on axon number and myelin sheath depth(P>0.05).Conclusion:Xenogeneic acellular nerve scaffold may be a promising approach in repairing peripheral nerve defect.关键词
异种/细胞外基质/支架/神经再生/免疫排斥反应Key words
Xenogeneic/Extracellular matrix/Scaffold/Nerve regeneration/Immunological rejection引用本文复制引用
陈少红,季婉青,侯博..异种源性脱细胞神经支架修复坐骨神经损伤后再生的可行性[J].中国医学创新,2016,13(20):27-32,6.基金项目
广东省自然科学基金（2014A030313189，2014A030310408）；广东省科技计划项目 （）
