中华耳科学杂志2016，Vol.14Issue(3)：408-413,6.DOI:10.3969/j.issn.1672-2922.2016.03.020
川芎嗪对庆大霉素耳聋豚鼠SOD同工酶蛋白表达的调控
Effects of TMP on Expression of SOD1, SOD2 and SOD3 in Cochlea of Guinea Pigs with Gentamycin-Induced Hearing Loss
沈晓丽 1周静 1王永华 2王一鸣 2顾伟忠3
作者信息
- 1. 宁波卫生职业技术学院 宁波315100
- 2. 浙江中医药大学 浙江310053
- 3. 浙江大学医学院附属儿童医院 浙江310003
- 折叠
摘要
Abstract
Objective To study the anti-oxidant effects of tetraethylplyrazine (TMP) on cochlea hair cells and spiral ganglion cells damaged by gentamycin (GM) and the relations between such effects and the expression level and activity of Cu/Zn superoxide dismutase (Cu/Zn SOD or SOD1), Mn-superoxide dismutase (Mn SOD or SOD2) and ECSOD (SOD3). Methods Sixty healthy guinea pigs were randomly divided into a model group (GM group), a GM+TMP treat-ment group, a TMP treatment group and a normal control group (n=15 in each group). Animals in the GM group were in-jected with Gentamycin sulfate at 120 mg/kg/d;those in the TMP group were injected with TMP at 30 mg/kg/d;those in the GM+TMP group were injected with Gentamycin sulfate and TMP with the same dosages;and those in the normal control group were injected with saline at 2.5 ml/kg/d. All animals were injected for 12 consecutive days. Before injec-tion and one day after the last injection, ABR thresholds were measured in all groups. At the end of the experiment, guin-ea pigs were sacrificed and immuno-histochemical methods were used to determine the level of SOD1, SOD2 and SOD3 protein expression in cochlear tissues. Results After treatment, ABR thresholds in the GM group were significantly high-er than the GM+TMP and normal control groups (P<0.01), while ABR thresholds in the TMP and normal control groups did not change significantly from before treatment (P>0.05). There were no significant differences in the expression of SOD1, SOD2 or SOD3 in spiral ganglion cells when the GM and GM+TMP groups were compared (P>0.05), but the ex-pression of SOD1 and SOD2 in hair cells was different between the two groups (P<0.05), although SOD3 expression in hair cells was not significantly different between the two groups (P>0.05). Conclusions GM is ototoxic and causes co-chlea hair cell damage. TMP plays an anti-oxidant role through regulation of SOD1, SOD2 expression in hair cells.关键词
庆大霉素/川芎嗪/耳毒性/抗氧化/SOD1/SOD2/SOD3Key words
GM/TMP/ototoxicity/anti-oxidant/SOD1/SOD2/SOD3分类
药学引用本文复制引用
沈晓丽,周静,王永华,王一鸣,顾伟忠..川芎嗪对庆大霉素耳聋豚鼠SOD同工酶蛋白表达的调控[J].中华耳科学杂志,2016,14(3):408-413,6.
