中华骨质疏松和骨矿盐疾病杂志2016，Vol.9Issue(2)：157-165,9.DOI:10.3969/j.issn.1674-2591.2016.02.009
17β-雌二醇及Wnt／β-catenin信号通路对人成骨肉瘤细胞基质Gla蛋白表达的影响
Effect of 17β-estradiol and Wnt/β-catenin signaling pathways on expression of matrix Gla protein in human osteosarcoma cells
摘要
Abstract
Objective To observe the effects of 17β-E2 and Wnt/β-catenin signaling pathways on the expres-sion of matrix Gla protein (MGP) in osteosarcoma cells (MG63), to investigate the potential role and the possible mechanism of MGP in osteoporosis.Methods MG63 cells were intervened by 17β-E2 (10 -10 , 10 -8 , 10 -6 ) mol/L, the inhibitor of estrogen receptor ICI 10 -7 mol/L for 48 h.MG63 cells were intervened by 17β-E2 10 -8 mol/L, (Dickkopf1) DKK-1 200 ng/mL, 17β-E2 10 -8 mol/L +DKK 1 200 ng/mL for 48 h.MG63 cells were inter-vened by 17β-E2 10 -8 mol/L, Warfarin 10 -5 mol/mL, 17β-E2 10 -8 mol/L +warfarin 10 -5 mol/mL for 48 h, then total RNA and protein was extracted.Real-time quantitative ( RT-PCR) and Western blotting were used to de-tectMGP,β-catenin,Runx2,LRP5geneandproteinexpression.Results 17β-E2couldup-regulatetheex-pression of MGP, at the concentration of 10 -10 , 10 -8 , 10 -6 mol/L.MGP mRNA expression were 4.63-fold, 7.16-fold, 2.95-fold, and MGP protein expression was 1.17-fold, 1.58-fold, 1.28-fold, compared with the control (P<0.05).At the concentration of 10 -8 mol/L, the effects of the 17 β-E2 was more obvious.The modu-lating effect of MGP by 17β-E2 was blocked after (Faslodex) ICI was used (P<0.05).17β-E2 increased the ex-pression of mRNA and protein of Wnt-associated proteinβ-catenin and LRP5.The Wnt/β-catenin signaling pathway inhibitor, DKK-1 could block the upregulation effect of MGP by 17β-E2 significantly ( P<0.05 ) .Warfarin de-creased the expression of MGP and block the upregulation effect of β-catenin, LRP5, Runx2 by 17β-E2 significant-ly ( P<0.05) .Conclusion It is maybe the mechanism of osteoporosis that 17β-E2 modulated the expression of MGP through Wnt/β-catenin signaling pathways.关键词
17β-雌二醇/骨肉瘤细胞/基质Gla蛋白/Wnt/β-catenin信号通路/骨质疏松症Key words
17β-estradiol/osteosarcoma cells/matrix Gla protein/Wnt/β-catenin signaling pathway/osteoporosis分类
医药卫生引用本文复制引用
杨雅,黄水金,谢菲飞,张洁,赖晓阳..17β-雌二醇及Wnt／β-catenin信号通路对人成骨肉瘤细胞基质Gla蛋白表达的影响[J].中华骨质疏松和骨矿盐疾病杂志,2016,9(2):157-165,9.基金项目
国家自然科学基金（81460171）；江西省教育厅基金 （）
