广东海洋大学学报2016，Vol.36Issue(4)：6-11,6.DOI:10.3969/j.issn.1673-9159.2016.04.002
基于cox1和D-loop区序列雷州半岛红树林海区2种野生弹涂鱼的遗传变异
Genetic Variation of 2 Wild Mudskipper Populations from Mangroves of Leizhou Peninsula Based oncox 1Gene and Control Region
摘要
Abstract
Double-cavern trap were designed tocapture wild mudskipper populations that could screen Periophthalmus modestusandBoleophthalmus pectinirostris populations to analyze sequence variation and genetic structurebased on mitochondrial control region (D-loop) andcox 1 gene sequence. The results showed that base composition of A + T was greater than that of C + G, which occurred on both cox 1 gene and the D-loop region. The data of cox 1 revealed 2.1%variation sites ofB. pectinirostris, whileP. modestushold2.4%. The averagegeneticdistance of intraspecificwas 0.003,0.006, respectively, and the interspecificwas0.158.Besides,37 individuals defined a total of 22 haplotypes based on cox 1 gene, whilehaplotype diversity was 0.95045.The dataofD-loop regionindicated thatB. pectinirostrishold 4.9%variation sitesof the total length andP. modestushad7.5%.The average geneticdistances of intraspecificandinterspecificwere 0.012,0.019 and0.409, respectively. Furthermore,37 individuals contained 32 haplotypes based on D-loop region. Haplotype diversity was at a very high level (0.98949).Neutral test results showed that evolution hypothesisof thetwo kinds mudskipperswereconsistent with neutral evolution, and population expansion might exist. Considering all these facts,it can be inferred that the genetic diversity ofP. modestuswas higherthanB. pectinirostris.关键词
红树林/弹涂鱼/捕捉器/序列变异/系统进化Key words
Mangroves/mudskipper/trapper/Sequence variation/Phylogenetic分类
农业科技引用本文复制引用
廖健,贺亮,张顺,沈自慧,黄永胜,龙水生,黄承勤,王中铎..基于cox1和D-loop区序列雷州半岛红树林海区2种野生弹涂鱼的遗传变异[J].广东海洋大学学报,2016,36(4):6-11,6.基金项目
广东海洋大学“海之帆-起航计划”大学生科技创新项目（hzfqhjhkjfm2015b11）；广东海洋大学优秀学位论文培育项目（201605）；国家自然科学基金项目(31201996) （31201996）
