广东海洋大学学报2016，Vol.36Issue(4)：17-22,6.DOI:10.3969/j.issn.1673-9159.2016.04.004
基于线粒体COI基因的雷州半岛马尾藻属常见种DNA条形码鉴定及系统进化分析
DNA Barcoding and Phylogenetic Analysis of Common Species of Sargassum from Leizhou Peninsula Based on mtDNA-COIGene Sequences
摘要
Abstract
Species identification and phylogenetic relationship in 6 common species ofSargassum collected from Leizhou Peninsula wereanalyzed byappling DNA barcoding technology assisted traditional morphological classification method.Mt-DNACOI gene sequencesof 6 species of Sargassum and existing data in GenBankand BOLD databasefor homology analysis showed that the similarity is99% or more,the molecular data were consistent withthe morphological identification results. In addition,theanalyses oncharacteristicsofCOI gene sequenceshows thatCOI gene sequence in 6speciesSargassumincluding 421 conserved sites, 58 variable sites, 21 Parsim-Informative sites, and 37 Singleton sites,accounted for 7.7%.Nucleartides composition results showed:T, C, A, G average contents were39.1%,18.9%,19.1%,22.9%, respectively, and A+T content (58.2%) was significantly higher thanC+Gcontent(41.8%).The highest frequency of amino acid was Phenylalanine (F) which coded by UUU(12.5%).The rate of transition and transversion based on maximum likelihood was2.42. Interspecific genetic distanceswerefrom 0.001±0.001to 0.113 ±0.016.The result ofcluster analysiswasconsistent withthat of morphological identification and homology analysis.关键词
马尾藻属/mtDNA-COI基因/物种鉴定/分子系统进化/DNA条形码Key words
Sargassum/mtDNA-COI/species identification/molecular phylogenetic/DNA barcoding分类
生物科学引用本文复制引用
贺亮,廖健,全秋梅,梁忠,谢恩义,刘丽,刘楚吾..基于线粒体COI基因的雷州半岛马尾藻属常见种DNA条形码鉴定及系统进化分析[J].广东海洋大学学报,2016,36(4):17-22,6.基金项目
2016年度广东大学生科技创新培育专项资金立项项目（pdjh2016b0236）；中央分成海域使用金支出项目（2011-2-2-09-3）；广东省海洋渔业科技推广专项（A201308E02）；广东省科技计划项目 （）
