广东海洋大学学报2016，Vol.36Issue(4)：37-44,8.DOI:10.3969/j.issn.1673-9159.2016.04.007
眼斑拟石首鱼烂尾病病原菌的分离鉴定及灭活疫苗的免疫效果
Isolation and Identification of Bacterial Pathogens from Sciaenops ocellatus with Tail-rotted Disease and Evaluation of Immune Efficacy of Inactived Vaccine
摘要
Abstract
A dominantbacterial strain designated as SoGZ1401 was isolated from ulcer of the moribund Sciaenops ocellatuswith rotted-tail disease and was confirmed to be the pathogen byartificial infection test. The 50% lethal dose of SoGZ1401 to theS.ocellatus was 8.6×103CFUg-1. Thestrain SoGZ1401 was identified asVibrio harveyi based onmorphology, physiological & biochemical tests and HSP60 DNA sequence analysis.The extracellular products ofV.harveyi strain were studied by cylinde-plate method. The results indicated that the extracellular products show the activities of amylase, gelatinase, casease, lipase, lecithinase and haemolysin, butwithout urease activity.Drug sensitivity tests revealed thatV.harveyi strain was sensitive to norfloxacin,Enrofloxacin, Florfenicol and compound sulfamethoxazole. The vaccine was prepared with formalin-inactivated strain SoGZ1401 to study antibody titer and immuneefficacy on S.ocellatus. The vaccination was administered by intraperitoneal injection and bath immersion route. Antibody titer in the serum of the injected group and immersion group were significantly increased compared with the control group and the maximal titers reach718.4 and 128,respectively. The Relative Percentage Survival (RPS)values of the injected group and immersion group were 75.0%-86.7%and 46.4%-56.7%, respectively.It is concludedthatS. ocellatus can produced strong immune response immunized withV.harveyibacterin, and the vaccine provide good protection efficiency against bacterial disease causeV.harveyi.关键词
眼斑拟石首鱼/烂尾病/病原/哈维氏弧菌/免疫效果Key words
Sciaenops ocellatus/tail-rotted disease/pathogen/Vibrio harveyi/immuneefficacy分类
农业科技引用本文复制引用
姚欢,黄郁葱,蔡双虎,鲁义善,蔡小辉,简纪常..眼斑拟石首鱼烂尾病病原菌的分离鉴定及灭活疫苗的免疫效果[J].广东海洋大学学报,2016,36(4):37-44,8.基金项目
广东省海洋经济创新发展区域示范专项（GD2012-B01-004）；广西海洋生物技术重点实验室开放基金(GLMBT-201404)；农业部农业公益项目（201203085）；广东省科技计划项目 （GLMBT-201404）
