广东海洋大学学报2016，Vol.36Issue(4)：45-49,5.DOI:10.3969/j.issn.1673-9159.2016.04.008
中草药与抗生素联用对罗非鱼源无乳链球菌的体外抑菌作用
Antibacterial Effectinvitro onStreptococcus agalactiae Isolated from Tilapia with Combination of Chinese Herbs and Antibiotics
摘要
Abstract
Theantibacterial activitiesof16 kinds of Chinese herbal medicine and 19 kinds of antibiotics were measured byjoan-board diffusion method, and the fractional inhibitory concentrations (FIC index) ofS. agalactiaefrom the combinations of3 herbal extracts and3antibiotics were determined by constant checkerboard test, andthe antibacterial activities of thecombination of Chinese herbs with antibiotics againstStreptococcus agalactiae isolated from tilapia, Oreochromis niloticus, were studied. The results showed that these16 kinds of herbal medicines and19 kinds of antibiotics presented good antibacterial effects onS. agalactiae, among them,and bothGalla chinensis andCoptis chinensiswater extract had the strongest effects of bacteriostasisin vitro, whose MIC were both3.9μg/mL, followed by Isatis tinctoriawith MIC250μg/mL. Cefazolin andCephalexinhad the strongest effects of bacteriostasisin vitro withMIC 0.125μg/mL, and followed byCarbenicillin (MIC 0.5μg/mL). Combination ofCoptis chinensis with Ceftriaxone, Cefazolin and Carbenicillin had a promoted antibacterialsynergy andadditive, and combination of,Galla chinensis with Carbenicillin showed antagonism,Radix Isatidis with three kinds of antibiotics showednon-correlation.关键词
罗非鱼/无乳链球菌/中草药/抗生素/微量抑制浓度指数Key words
Oreochromis niloticus/Streptococcus agalactiae/Chinese herbal medicine/Antibiotic/Fractional inhibitory concentrations(FIC)分类
农业科技引用本文复制引用
李焯新,蔡小辉,黄瑜,王蓓..中草药与抗生素联用对罗非鱼源无乳链球菌的体外抑菌作用[J].广东海洋大学学报,2016,36(4):45-49,5.基金项目
广东省科技计划项目（2013B020307014），广东海洋大学优秀青年骨干教师特别资助计划 （）
