广东海洋大学学报2016，Vol.36Issue(4)：66-72,7.DOI:10.3969/j.issn.1673-9159.2016.04.011
不良环境对溶藻弧菌胱硫醚-β-合成酶活力及抗氧化系统的影响
Influence on Cystathionine-β-Synthase and Antioxidant System of Vibrio alginolyticus under Adverse Environment
摘要
Abstract
The influences on the activity of cystathionine-β-synthase (CBS), superoxide dismutase (SOD), peroxidase (POD), and content of glutathione (GSH) ofVibrio alginolyticus under the conditions of unsuitable pH, temperature, salinity or antibiotic stress and exogenous hydrogen sulfidedonor NaHS were studied, and the correlation between CBS and antioxidant system was analyzed. The result shows that the activity of CBS was improved significantly under the unsuitable temperature and antibiotic stress than controls(P<0.05). The activity of SOD, POD and the content of GSH were also upregulated on the condition of unsuitable temperature(P<0.05). Under the stress of erythromycin and spectinomycin, the activity of SOD and POD were upregulated(P<0.05), and gentamicin and tracyclin could also improve the activity of SOD and POD(P<0.05), respectively. Under the condition of unsuitable pH, low pH not only improved the vitality of CBS but also upregulated SOD, POD activity and GSH content(P<0.05), whereas the vitality of CBS did not show significant difference to the control and SOD activity down-regulated(P<0.05) under high pH. On the condition of unsuitable salinity, low and high salinity could also improve GSH content, while the activity of CBS was increased only in the treatment of unsuitable salinity(P<0.05). After the treatment of antibiotics+NaHS,CBS activity were not improved significantly(P>0.05), while SOD activity and GSH content were upregulated (P<0.05). Correlation analysis indicated that there was positive correlation between CBS activity and SOD, POD activity or GSH content under different temperature, salinity and antibiotic stress(P<0.05), whereas CBS activity and SOD, POD activity or GSH content under the condition of different pH and antibiotics+NaHS were not significantly correlative (P>0.05). In conclusion, improved CBS can helpV.alginolyticus to resist the adverse environmental by adjusting antioxidant system. Exogenous hydrogen sulfide can directly improve the vitality of SOD and GSH content ofV.alginolyticus to maintain its normal physiological activities.关键词
不良环境/溶藻弧菌/胱硫醚-β-合成酶/抗氧化系统Key words
adverse environment/Vibrio alginolyticu/cystathionine-β-synthase/antioxidant system分类
生物科学引用本文复制引用
陈树河,常云胜,刘晖晖,周维,丁燏..不良环境对溶藻弧菌胱硫醚-β-合成酶活力及抗氧化系统的影响[J].广东海洋大学学报,2016,36(4):66-72,7.基金项目
广东省自然科学基金(2014A030313604)；广东海洋大学“创新强校工程”2013年省奖补资金支持建设项目--高校重大科研项目培育计划（第3类）（Q14196(2013050207)）；广东省教育厅高等学校高层次人才项目“谷胱甘肽及其合成酶系在哈氏弧菌耐药中的作用机制研究”。 （2014A030313604）
