中南民族大学学报（自然科学版）2016，Vol.35Issue(2)：51-55,5.
肿瘤微环境酸化下 MDSCs通过 ASICs 促进 TNF-α表达
Promoted TNF-αExpression of MDSCs by ASICs under Acidic Tumor Microenvironment
摘要
Abstract
To investigate the relationship between the TNF-αexpression and ASICs in MDSCs under the tumor microenvironment , real-time PCR was performed to determine the TNF-αexpression changes in mice under normal physiological (pH 7.3), acidic (pH 5.5), and inhibitor-added condition, respectively.The results indicated that the expression of TNF-αsignificantly elevated in tumor MDSCs under normal physiological condition as well as acidic condition.In contrast, the expression was significantly inhibited by the addition of ASICs′nonspecific inhibitors, amiloride, diclofenac, or ASICs subunit 1 inhibitor PcTx-1.However, pretreatment with ibuprofen or ASIC subunits 3 inhibitors APETX2 had no effect .So the ASICs subunit 1 of MDSCs was likely responsible for promoting the expression of TNF-αunder the acidic tumor microenvironment .关键词
髓源抑制细胞/肿瘤坏死因子/荧光定量PCR/酸敏感离子通道/抑制剂Key words
MDSCs/TNF-α/real-time PCR/ASICs/inhibitor分类
医药卫生引用本文复制引用
胡鑫,伊萍,明莹,童胜兰,龚惠红..肿瘤微环境酸化下 MDSCs通过 ASICs 促进 TNF-α表达[J].中南民族大学学报（自然科学版）,2016,35(2):51-55,5.基金项目
国家自然科学基金资助项目（81201610）；湖北省民族药物现代化工程技术研究中心（中南民族大学）资助项目（2015ZY008）；中南民族大学本科教学工程项目 （）
