作物学报2016，Vol.42Issue(7)：957-965,9.DOI:10.3724/SP.J.1006.2016.00957
水稻斑马叶突变体zebra1349的表型鉴定及基因精细定位
Morphological Characterization and Fine Mapping of Zebra Leaf Mutant zebra1349in Rice (Oryza sativaL.)
摘要
Abstract
A new zebra leaf mutant zebra1349was attained in a restorer line crossing population of [R128//(R318/R1025) F1] F6 in Hengyang Agricultural Science Research Institute of Hunan province. This mutant showed normal green leaves at seedlings stage, but a zebra leaf phenotype with green-yellow bands in penpendicular to leaf vein appeared at five days after transplanting, which was most obvious at sixth-leaf stage, and recovered normal green leaves around 30 days (ninth-leaf stage) after transplant-ing. Until the mature stage, the zebra1349 mutant showed insignificant difference with the wild type in major agronomic traits. The contents of total chlorophyll, chlorophylla, chlorophyllb and carotenoid in yellow parts of the mutant leaf at sixth-leaf stage decreased by 55.86%, 61.02%, 39.34%, and 47.03%, respectively. Transmission Electron Microscopic (TEM) results indicated that the chloroplast of the mutant yellow leaf showed a serious thylakoid membrane degradation and decomposition, and the number of thylakoid grana lamella decreased significantly with larger gap and looser arrangement. Genetic analysis using F1and F2of the reciprocal crosses betweenzebra1349 and normal green rice varieties revealed that the zebra-leaf trait was controlled by one pair of recessive nuclear genes. With 1192 recessive plants in a F2population from the cross betweenzebra1349 mutant and normal green variety 02428, theZEBRA1349gene was finely mapped between two InDel markers indel39 and indel44 on chro-mosome 12 with a genetic distance of 0.04 cM and 0.17 cM respectively, and the physical distance was 89 kb based on comparing with the reference genome of Japonicarice Nipponbare. These results provide a foundation for further map-based cloning of ZEBRA1349 and molecular marker-assisted breeding.关键词
水稻(Oryza sativaL.)/斑马叶突变体/叶绿体/基因精细定位Key words
Rice (Oryza sativa L.)/Zebra leaf mutant/Chloroplast/Gene fine mapping引用本文复制引用
郭国强,孙学武,孙平勇,尹建英,何强,袁定阳,邓华凤,袁隆平..水稻斑马叶突变体zebra1349的表型鉴定及基因精细定位[J].作物学报,2016,42(7):957-965,9.基金项目
本研究由国家高技术研究发展计划(863计划)项目“强优势水稻杂交种的创制与应用”(2011AA10A101)和衡阳市科技局重点项目“黄色带状标记基因在两用核不育系中的利用”(2011KZ15)资助。 The study was supported by the grants from National High-tech R&D Program of China (863 Program)(2011AA10A101) and the Key Project Funded by the Hengyang Science and Technology Bureau (2011KZ15) （863计划）
