作物学报2016，Vol.42Issue(7)：966-975,10.DOI:10.3724/SP.J.1006.2016.00966
一个水稻显性斑点叶突变体的鉴定和基因精细定位
Characterization and Gene Fine Mapping of a Rice Dominant Spotted-leaf Mutant
摘要
Abstract
A stable inherited rice spotted-leaf mutantHM113 was isolated from an EMS-induced IR64 mutant bank. Under natural conditions, brown lesions were observed on the leaves in three weeks after sowing and spread to the sheaths at the initial heading stage. Agronomic traits including the plant height, panicle length, number of panicles, number of filled grain/panicle, seed-setting rate and 1000-grain weight were decreased significantly inHM113. In addition, the photosynthetic pigment contents, net photo-synthetic rate and soluble protein content in the mutant were significantly lower than those in the wild type IR64, while the MDA content was similar to that in the wild-type. Activities of CAT and SOD were significantly lower and activity of POD was signifi-cantly higher in the mutant than in IR64. Histochemical analysis showed that cell death and ROS accumulation were occurred in and around the lesions inHM113. Furthermore, disease resistance to bacterial blight pathogens was significantly enhanced in the mutant in contrast to that in the wild type IR64. Expression of defense-related genes includingAOS2,PAL4,PR10,andPR1b was apparently up-regulated in the mutant. Genetic analysis indicated that the mutant trait was controlled by a novel single dominant nuclear gene, tentatively termedas SplHM113, which was detected to be located in a region around 308 kb flanked by RM21605 and RM418 on the long arm of chromosome 7. The data and populations obtained in the present study would facilitate the isolation and functional analysis ofSplHM113.关键词
水稻/斑点叶突变体/白叶枯病抗性/活性氧/基因定位Key words
Rice/Spotted-leaf mutant/Bacterial blight resistance/Reactive oxygen species/Gene mapping引用本文复制引用
郭丹,施勇烽,王惠梅,张晓波,宋莉欣,徐霞,贺彦,郭梁,吴建利..一个水稻显性斑点叶突变体的鉴定和基因精细定位[J].作物学报,2016,42(7):966-975,10.基金项目
本研究由浙江省自然科学基金项目(LQ15C130005)和国家高技术研究发展计划(863计划)项目(2014AA10A603-15)资助。 This study was supported by the Natural Science Foundation of Zhejiang Province (LQ15C130005) and the National High-tech R&D Program of China (2014AA10A603-15) （LQ15C130005）
