作物学报2016，Vol.42Issue(7)：976-983,8.DOI:10.3724/SP.J.1006.2016.00976
水稻早衰突变体esl6的鉴定与基因定位
Identification and Gene Mapping of an Early Senescent Leaf Mutantesl6 in Oryza sativa L
摘要
Abstract
As an essential process in life, natural senescence is necessary to adapt plant to environment diversity, while earlier senescence could reduce yield per unit and cause inferior quality in crop production. Therefore, it is significant to elucidate senes-cence molecular mechanism in plant. Here, we reported a novel rice mutantesl6derived from the progeny of EMS-induced re-storer line Jinhui 10, which senescent peculiarity was observed at the early stage of life. In detail, cultivated under the paddy field, theesl6 had no obvious difference with the wild type before the 4-leaf stage, while after that the whole leaf blade ofesl6 displayed chlorosis in the tip and kept normal green in the base until the flowering stage. Subsequently, all leaf blades in theesl6 demon-strated chlorosis and senescence, still more severe at the upper position. Observation by scanning electron microscope showed that cell structures in the senescent location ofesl6 leaf blade were abnormal and filled with ruptured cell membranes, enlarged vacu-oles and broken organelles such as the chloroplasts containing incomplete stroma thylakoids and excessive starch grains. Mean-while, early senescence significantly lessened photosynthetic pigment contents and photosynthetic rate. The activities of SOD, CAT, and POD raised and the contents of O2܋, H2O2, and ·OH increased in the esl6 leaf tip, and all of the differences led to the extremely significant level compared with those of the wild type. Additionally, the mutational plant showed semi-dwarfism and shorter leaf blades, the first and second internodes decreased to the extremely significant level in statistics. Genetic analysis sug-gested that the mutational traitswere controlled by a recessive nuclear gene. The gene was finally mapped on chromosome 9 with 203 kb physical distances between Indel markers Sind09-3 and Sind09-4 on the basis of F2 generation of Xida1A/esl6. All of these provide a foundation for ESL6 cloning and function analysis and then are beneficial to ascertaining the molecular mechanism of senescence inOryza sativaL.关键词
水稻(Oryza sativa L.)/早衰/基因定位Key words
Rice (Oryza sativaL.)/Early senescent leaf blades/Gene mapping引用本文复制引用
杨波,夏敏,张孝波,王晓雯,朱小燕,何沛龙,何光华,桑贤春..水稻早衰突变体esl6的鉴定与基因定位[J].作物学报,2016,42(7):976-983,8.基金项目
本研究由中央高校基本科研业务费(XDJK2013A023)和国家自然科学基金项目(31171178)资助。 This study was supported by the Fundamental Research Funds for the Central Universities (XDJK2013A023) and the National Natural Science Foundation of China (31171178) （XDJK2013A023）
