作物学报2016，Vol.42Issue(7)：984-989,6.DOI:10.3724/SP.J.1006.2016.00984
小麦未减数配子基因的连锁标记及染色体区段检测
Detection of the Molecular Marker and Chromosomal Segment linked to Un-reduced Gamete Gene in Common Wheat
摘要
Abstract
Hexaploid common wheat (Triticum aestivumL., AABBDD, 2n= 42) arose from spontaneous chromosome doubling of the hybrid betweenT. turgidumandAegilops tauschiiCosson. The process of chromosomes doubling is mainly determined by unreduced gametes (UG) genes inT. turgidum. The genetic effects on the UG production may vary amongT. turgidum lines. In this study, a SSR marker close to the UG geneQTug.sau-3B(Xgpw1146) and high throughput DArTseq genotyping technique were used to screen the UG gene in common wheat lines transferred fromT. turgidum via synthetic hexaploid wheat (SHW) as a bridge. Out of the analyzed 105 SHW-derived elite lines, 17 had theXgpw1146 allele fromT. turgidum, indicating that the UG gene was probably transferred into these wheat lines. According to the DArTseq genotyping data on 88 lines derived from the synthetic hexaploid wheat SHW-L1, all these lines with theT. turgidumXgpw1146 allele contained a chromosomal segment of SHW-L1, probably covering theXgpw1146 locus. This indicates that the adjacent region of the UG gene as a chromosomal segment was trans-ferred into wheat lines. These SHW-derived lines have important application potential on wheat doubled haploid breeding.关键词
异源多倍体/人工合成小麦/未减数配子Key words
Allohexaploid/Synthetic wheat/Unreduced gamete引用本文复制引用
寇春兰,赵来宾,刘梦,郝明,甯顺腙,袁中伟,刘登才,张连全..小麦未减数配子基因的连锁标记及染色体区段检测[J].作物学报,2016,42(7):984-989,6.基金项目
本研究由国家自然科学基金项目(31271723,31201210),四川省杰出青年基金项目(2011JQ0016)和四川省教育厅科研项目(14ZA0012)资助。 This study was supported by the National Natural Science Foundation of China (31271723,31201210), Sichuan Provincial Youth Fund (2011JQ0016), and the Scientific Research Foundation of the Education Department of Sichuan Province (14ZA0012) （31271723,31201210）
