作物学报2016，Vol.42Issue(7)：990-999,10.DOI:10.3724/SP.J.1006.2016.00990
大豆盐胁迫相关GmNAC基因的鉴定、表达及变异分析
Identification, Expression and Variation Analysis of Salt Tolerance Related GmNAC Genes in Soybean
摘要
Abstract
NAC genes play an important role in plant stress tolerance. In this study, bioinformatic method was used to identify the stress relatedGmNAC gene in soybean. The expression of candidatedGmNAC genes in root and leaf was analyzed in soybean with NaCl treatment by Real-time PCR. Reverse transcription PCR was performed to clone genes with significant difference in expression. The results showed that there were 175 genes in soybeanGmNAC gene family. There were 11 GmNACproteins with highly conserved NAC located on the same evolutionary branch with the stress related NAC proteins in rice and Arabidopsis. The expression of 11GmNAC genes in soybean root was higher than that in leaf. TheGmNAC genes were all induced by NaCl stress, but part of theGmNAC genes showed different expression levels between root and leaf in soybean varieties with different salt tolerances. There were three synonymous mutations and one non-synonymous mutation on the CDS region ofGlyma06g11970.1 and one synonymous mutation on the CDS region ofGlyma06g16440.2 in Qihuang 34, Xudou 10, and Fendou 95.关键词
大豆/GmNAC/进化树/NaCl处理/表达分析/序列变异Key words
Soybean/GmNAC/Phylogenetic tree/NaCl treatment/Expression analysis/Sequence variation引用本文复制引用
张彦威,张礼凤,李伟,王彩洁,张军,徐冉..大豆盐胁迫相关GmNAC基因的鉴定、表达及变异分析[J].作物学报,2016,42(7):990-999,10.基金项目
本研究由?东北农业大学大豆生物学教育部重点实验室开放基金项目(SB14A04),国家现代农业产业技术体系建设专项(CARS-04- CES18),国家自然科学基金项目(31501329)和山东省自然科学基金项目(ZR2015YL070)资助。 The study was supported by the Open Foundation of Key Laboratory of Soybean Biology of Ministry of Education, Northeast Agricultural University (SB14A04), the China Agriculture Research System (CARS-04-CES18), the National Natural Science Foundation of China (31501329), and the Natural Science Foundation of Shandong Province (ZR2015YL070) （SB14A04）
