作物学报2016，Vol.42Issue(7)：1067-1073,7.DOI:10.3724/SP.J.1006.2016.01067
小麦花发育MADS-box基因的表达模式分析
Expression Patterns ofMADS-box Genes Related to Flower Development of Wheat
摘要
Abstract
The objective of this study was to elucidate the molecular mechanism of wheat (Triticum aestivum L.) flower develop-ment. According to the phylogenetic tree ofMADS- box genes from different species, we found that wheat contained all kinds of genes involved in the ABCDE model for flower development. The expression patterns of A-, B-, C-, D-, and E-class genes were analyzed by semi-quantitative and quantitative RT-PCR (qRT-PCR). WheatAP1/FUL geneTaFUL (A-class) was expressed in all floral organs with the highest expression level in lemmas and paleas. GenesTaAP3 (B-class),TaAG(C-class) showed conservative expression patterns in specific organs, i.e.,TaAP3 was expressed in lodicules and stamens whereasTaAGwas expressed in sta-mens and pistils. TheOsMADS13 homologous gene in wheat (D-class) was expressed in both pistils and lodicules, suggesting its function in lodicule and ovule development simultaneously. GeneTaSEP (E-class) was mainly expressed in paleas and the in-ner-three whorls. LHS1 is a grass-specific gene family and belongs to E-class. The expression ofTaLHS1 was detected in lemmas, paleas, and glumes of wheat.TaDL, the homologous gene of rice DROOPING LEAF (DL) controlling carpel development, was expressed in glumes, lemmas and carpels. These results suggest a conservative molecular mechanism for flower development in wheat, but some genes may have diversified functions due to evolution. The expression evidence ofTaDL andTaLHS1 in glumes, in combination with the morphology and structure analyses of glume, lemma and palea, implied that lemma and glume might originate from the same organ in wheat.关键词
小麦/花发育/MADS-box基因/表达模式Key words
Wheat/Flower development/MADS- box gene/Expression pattern引用本文复制引用
李海峰,韩英,刘梦佳,王冰华,苏亚丽,孙其信..小麦花发育MADS-box基因的表达模式分析[J].作物学报,2016,42(7):1067-1073,7.基金项目
本研究由国家自然科学基金项目(31571657),中央高校基本科研业务费项目(2014ZZ009)和新疆农业职业技术学院科研项目(XJNZYKJ201501)资助。 This study was supported by the National Natural Science Foundation of China (31571657), the Fundamental Research Foundation for the Central Universities (2014ZZ009), and the Foundation of Xinjiang Agriculture Vocational Technology College (XJNZYKJ201501) （31571657）
