作物学报2016，Vol.42Issue(7)：1094-1099,6.DOI:10.3724/SP.J.1006.2016.01094
花生AhDGAT2a基因启动子的克隆和功能验证
Cloning and Functional Analysis of PeanutAhDGAT2a Promoter
摘要
Abstract
Diacylglycerol acyltransferase (DGAT) is a rate-limiting enzyme in triacylglycerol (TAG) biosynthesis pathway. In this study, GenomeWalking method was used for cloning the promoter sequence ofAhDGAT2a gene from Luhua 14, and finally a 1200 bp fragment flanking 5′-upstream ofAhDGAT2a was obtained and named as pAhDGAT2a. The crucial regulatory elements in pAhDGAT2a were further analyzed with software PlantCARE. There were many TATA-box, CAAT-box, light regulation, stress and defense response and hormone response elements. To assess the activity of pAhDGAT2a, we constructed pAhDGAT2a:GUS cassettes and introduced it into the tobacco SR1 genome by Agrobacterium-mediated transformation. Expression pattern was monitored by histochemical staining. Results showed that GUS activity driven by the pAhDGAT2a was detected in almost all vegetative and reproductive tissues, with a higher expression level in stigma, anther and young seeds than in the other organs, indicating thatpAhDGAT2a has a constitutive promoter activity.关键词
花生/AhDGAT2a基因/启动子/功能验证Key words
Arachis hypogaeaL./AhDGAT2a gene/Promoter/Function analysis引用本文复制引用
郑玲,史灵敏,田海莹,单雷,边斐,郭峰,宣宁,万书波,彭振英..花生AhDGAT2a基因启动子的克隆和功能验证[J].作物学报,2016,42(7):1094-1099,6.基金项目
本研究由山东省自然科学基金项目(ZR2013CM036)和山东省良种工程项目(2014-2017)资助。 This study was supported by Natural Science Foundation of Shandong (ZR2013CM036) and Shandong Province Germplasm Innovation and Utilization Project (2014-2017) （ZR2013CM036）
