摘要
Abstract
Objective To study the chemiluminescence immunoassay kit for the development of TSH in human serum.Methods Horseradish peroxidase labeled anti TSH subunit specific monoclonal antibody (mAb),and anti TSH subunit of another mAb pairs,using luminol as a substrate,took two steps to establish a two-site sandwich method to carry out research in human serum TSH chemiluminescence immune detection kit.Results This method had a good sensitivity,in which the average intra-assay CV was 4.7％,and the average betweenrun CV was 8.4％.The detection experiment and LH,FSH,HCG had no cross reaction.After the test part of the experimental samples,the results obtained from the reagent and similar reagents in foreign countries had obvious correlation (r=0.984).Conclusion The detection method of experimental study has good sensitivity and accuracy,is relatively simple,fast,low-cost compared with similar products,worth to use in clinical testing and research.关键词
TSH/化学发光免疫检测/试剂盒Key words
TSH/Chemiluminescence immunoassay/Kit
