广西科学2016，Vol.23Issue(3)：234-240,7.DOI:10.13656/j.cnki.gxkx.20160713.014
热提羊栖菜多糖对黑腹果蝇生长的影响
Effects of Polysaccharides from Sargassum fusiforme on Growth of Drosophila melanogaster
摘要
Abstract
Objective]The effects of different concentrations of Sargassumfusiforme polysaccar-ides (SFPs)extracted by heat extraction and alcohol precipitation on growth of Drosophila melanogaster were investigated.[Methods]The SFPs were extracted through 85℃ hot boiled extract,ethanol precipitation,calcium chloride to remove impurities and other processes.The effects on larval emergence,weight and body size of Drosophila melanogaster were explored in different concentrations of the SFPs in the media.[Results]The SFPs obtained by heat extrac-tion and alcohol precipitation had high purity,total sugar content as high as 81.93%,molecular weight and monosaccharide composition revealed more stable.Drosophila melanogaster increased in size and weight after treatment with SFPs. Compared with the control group,There was statistical difference in 0.1%♀ and 1.5%♀groups(P <0.05 )and there was significantly different between 0.5%♀ and control group;The experimental groups of 0.1% ♂,0.5% ♂and 1 .5% ♂ revaeled significant differences with the control group,but no significant difference between them,of which weight gain was most evident in 1 .5%♂ group.There were significant differences in the larvae hatching be-tween experimental group and control group;The phase from egg to feathering was 5~8 d in control group,6~9 d in both 0.1% and 0.5% groups,and 6~10 d in 1.5% group.There was a significant difference in emergence rate in 0.1%♀,1.5%♀ and 0.1%♂ groups compared with the corresponding control group.Considering the proportion of male and female adult e-mergence,only 1 .5% group significantly differed with CK group.[Conclusion]The SFPs ob-tained by heat extraction and alcohol extraction are stable.They can reduce the larval emer-gence rate and prolong larval emergence time.They have a little effect on the proportion of male and female,and can increase the adult body and weight.关键词
羊栖菜多糖(SFPs)/黑腹果蝇/羽化率/体型/体重Key words
SFPs/Drosophila melanogaster/emergence rate/body size/weight分类
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张亚,肖保衡,吴明江..热提羊栖菜多糖对黑腹果蝇生长的影响[J].广西科学,2016,23(3):234-240,7.基金项目
国家自然科学基金项目(31470430,31200266,31070322)和温州市海洋环境与生物资源技术创新团队自主项目(C20120007-10)资助。 （31470430,31200266,31070322）
