广西植物2016，Vol.36Issue(7)：763-767,5.DOI:10.11931/guihaia.gxzw201412005
油菜素内酯对巨桉组培中不定根诱导、苗木生长和茎基部细胞结构的影响
Adventitious root induction, shoot height and cellular structure of basal stem by exogenous application of brassinosteriod in Eucalyptus grandis
摘要
Abstract
Brassinosteroids ( BRs) refer to a group of polyhydroxylated plant steroid hormones that are essential regula-tors of plant architecture, growth and development including embryogenesis, cell division, vascular system differentiation and stress tolerance processes. However, the effects of BRs in adventitious root induction of Eucalyptus have not been demonstrated. In this study, adventitious root induction, growth of shoots and histochemical staining of basal stem were <br> analyzed by adding the exogenous brassinosteriod (Concentration:0, 0.005, 0.01, 0.05, 0.10, 0.20 mg.L-1,respec-tively) in the root inducing medium of E. grandis clones EG5 and GL1. The results showed that the height of shoot and induction frequency of adventitious root of E. grandis clone EG5 were significantly affected by adding the exogenous bras-sinosteriod. Induction frenquency of adventitious root was 76.6%. The length of adventitious root of E. grandis clone EG5 was the highest in the root inducing medium containing 0.005 mg.L-1 brassinosteriod for. In the meantime. The height of shoot decreased significantly with the increasing concentration of exogenous brassinosteriod and it was reduced by 21.8%at 0.10 mg.L-1 brassinosteriod concentration compared to control. The adventitious root induction frequency of E. gran-dis clone GL1 reached the highest level with 88.3% in the root inducing medium of adding 0.05 mg.L-1 brassinosteriod concentration but the height of shoot showed no significant change by adding the exogenous brassinosteriod. It was inter-esting that the root elongation of E. grandis clone EG5 was significantly inhibited due to the addition of the exogenous brassinosteriod. Root length and number were reduced to 2.49 cm and 1.75 at 0.20 mg.L-1 brassinosteriod from 4.64 cm and 3.02( control) respectively. However, the lateral root induction and differentiation were not affected by adding the exogenous brassinosteriod. Besides, the growth in xylary cells of basal stem was clearly promoted and the cambium cell number of basal stem increased at 0.10 mg.L-1 brassinosteriod by way of the histochemical analysis. Together, brassi-nosteriods was necessary to adventitious root induction and shoot growth and increasing xylem was a primer cause of lower frenquency of adventitious root induction at a few extra brassinosteriod.关键词
油菜素内酯/桉树/不定根诱导/形成层Key words
brassinosteriod/Eucalyptus grandis/adventitious root induction/cambium分类
农业科技引用本文复制引用
闫慧芳,曾炳山,范春节,刘英,裘珍飞,李湘阳..油菜素内酯对巨桉组培中不定根诱导、苗木生长和茎基部细胞结构的影响[J].广西植物,2016,36(7):763-767,5.基金项目
国家自然科学基金(31400554) （31400554）
国家科技计划项目(2013AA102705) （2013AA102705）
中央级公益性科研院所基本科研业务费专项(RITFYWZX201304)[Supported by the National Natural Science Foundation of China(31400554) （RITFYWZX201304）
Project of National Science and Technology Plan (2013AA102705) （2013AA102705）
Special Foundamental Research Fund of the Central Research Institutes of Public Benefit (RITFYWZX201304)]。 （RITFYWZX201304）
