海洋科学2016，Vol.40Issue(5)：19-28,10.DOI:10.11759/hykx20151030001
刺参4个不同选育品系幼参对低盐胁迫的耐受及生理生化响应
Response to low salinity of four strains of sea cucumber Apos-tichopus japonicus larvae
摘要
Abstract
The salinity of the sea in estuarine areas is low and unstable, which threatens the survival and growth of sea cucumbers. To evaluate the low salinity tolerance of four sea cucumber (Apostichopus japonicus) strains (RC-F2, KC-F3, DC-F2, and QQ-F1), the growth, survival, and non-specific immune enzyme activities were monitored at seven salinity levels (13, 14, 15, 16, 17, 18, and 31); wild sea cucumber specimens from the Qingdao coast (QW) were used as controls. TheRSGs (Specific Growth Rate) of all the strains, including the control, QW, decreased sig-nificantly under the stress of low salinity conditions. Only the QQ-F1 strain showed positiveRSG at salinity 17. The LS50-30d of QQ-F1 was significantly lower than that of the other strains (RC-F2, KC-F3, DC-F2, QQ-F1, and QW were 15.02, 15.19, 16.48, 14.26, and 15.13, respectively). At salinity 14, the ST50 of QQ-F1 was significantly longer than that of the other strains (RC-F2, KC-F3, DC-F2, QQ-F1, and QW were 19.84d, 18.43d, 10.11d, 23.54d, and 19.01d, respectively). Although the enzyme activities of AKP, ACP, SOD, and LZM were decreased in all 5 species under low salinity conditions (salinity<18), the QQ-F1 strain maintained a comparatively higher enzyme activity level and showed higher overall tolerance to the stress of low salinity. Based on the growth, survival, and non-specific immune enzyme activities, the tolerance to low salinity of the 5 populations was ordered as follows: QQ-F1>RC-F2>QW>KC-F3>DC-F2. The high tolerance of QQ-F1 for low salinity suggest that it would be a good candidate for popularization of low salinity sea areas or unstable salinity coastal areas. These results could be help-ful for healthy farming and breeding of sea cucumbers, as well as for evaluations of their character.关键词
刺参(Apostichopus japonicus)/低盐胁迫/特定生长率/半致死盐度/半致死时间/非特异性免疫酶Key words
Apostichopus japonicus/low salinity stress/specific growth rate/median lethal salinity/median survival time/non-specific immune enzyme activity引用本文复制引用
侯西坦,廖梅杰,李彬,王印庚,张正,陈贵平,荣小军,孙金生,范瑞用..刺参4个不同选育品系幼参对低盐胁迫的耐受及生理生化响应[J].海洋科学,2016,40(5):19-28,10.基金项目
国家十二五“863”项目(2012AA10A412-4) （2012AA10A412-4）
国家自然科学基金项目(31202016) （31202016）
山东省农业良种工程重大项目“速生抗病耐高温刺参良种选育” （）
国家948项目(2014-Z13) （2014-Z13）
山东省自主创新成果转化专项(2013ZHZX2A0801)@@@@National High Technology Research and Development Pro-gram, China, No.2012AA10A412-4 （2013ZHZX2A0801）
National Natural Science Foundation of China, No.31202016 （）
Agriculture Seed Improvement Project of Shan-dong Province （）
Program for international advanced agricultural science and technology introduction, No.2014-Z13 （）
Special Research Funds for Independent Innovation and Scientific & Technology Achievements Transformation Of Shandong Province, No.2013ZHZX2A0801。 （）
