海洋科学2016，Vol.40Issue(5)：29-35,7.DOI:10.11759/hykx20150824001
企鹅珍珠贝Creb2基因的克隆及表达分析
Molecular cloning and expression analysis ofCreb2gene from Pteria penguin
摘要
Abstract
In this study, in order to analyze the sequence and expression characteristics of the cAMP response ele-ment binding protein (Creb) gene in thePteria penguin, we characterized the full-length cDNA of theCreb gene from thePteria penguinby the rapid amplification of cDNA ends (RACE)-polymerase chain reaction (PCR). We then used real-time PCR to examine the expression ofCreb in different tissues.The results show that the full-length cDNA ofCreb was 1484 bp, including a 5¢UTR of 218 bp, a 3¢UTR of 195 bp, and an open-reading frame of 1071 bp, which encodes a deduced protein of 356 amino acids. The C-terminal region contained a conserved basic region leucin zipper (bZIP). The predicted molecular weight was 39.49 kD, and the isoelectric point was 4.43. The se-quence comparison showed thatCreb in thePteria penguin(ppCreb2) shares 46.3% and 46.1% sequence identities withCreb2in Ostrea edulis andCrassostrea gigas, respectively.The phylogenetic analysis results were consistent with traditional taxonomic analysis. The real-time PCR showed thatppCreb2was constitutively expressed in all studied tissues (mantle, gill, adductor muscle, digestive diverticulum, foot, testis, and ovary), with higher levels in the foot and gill.关键词
企鹅珍珠贝/ppCreb2/基因克隆/表达分析/荧光定量PCRKey words
Pteria penguin/ppCreb2/Gene cloning/expression analysis/real-time PCR分类
生物科学引用本文复制引用
于非非,余祥勇,潘珍妮,宋娜娜,王梅芳..企鹅珍珠贝Creb2基因的克隆及表达分析[J].海洋科学,2016,40(5):29-35,7.基金项目
广东海洋大学优秀青年骨干教师培养项目(20140040) （20140040）
广东海洋大学博士启动基金(E15041) （E15041）
广东省海洋渔业科技推广专项项目(A201308A11)@@@@the Outstanding Young Teacher Foundation of Guangdong Ocean University, No.20140040 （A201308A11）
Doctoral research project of Guangdong Ocean University, No.E15041 （）
the Technology Extension Foundation of Marine Fishery in Guangdong Province, No.A201308A11。 （）
